Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine

Ganna Aleshcheva; Jayashree Sahana; Xiao Ma; Jens Hauslage; Ruth Hemmersbach; Marcel Egli; Manfred Infanger; Johann Bauer; Daniela Gabriele Grimm

doi:10.1371/journal.pone.0079057

Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine

Ganna Aleshcheva, Jayashree Sahana, Xiao Ma, Jens Hauslage, Ruth Hemmersbach, Marcel Egli, Manfred Infanger, Johann Bauer, Daniela Gabriele Grimm

Institut for Biomedicin - Forskning og uddannelse, Øst

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

64 Citationer (Scopus)

Abstract

Tissue engineering of chondrocytes on a Random Positioning Machine (RPM) is a new strategy for cartilage regeneration. Using a three-dimensional RPM, a device designed to simulate microgravity on Earth, we investigated the early effects of RPM exposure on human chondrocytes of six different donors after 30 min, 2 h, 4 h, 16 h, and 24 h and compared the results with the corresponding static controls cultured under normal gravity conditions. As little as 30 min of RPM exposure resulted in increased expression of several genes responsible for cell motility, structure and integrity (beta-actin); control of cell growth, cell proliferation, cell differentiation and apoptosis (TGF-β1, osteopontin); and cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours of RPM exposure disruptions in the vimentin network were detected. These changes were less dramatic after 16 hours on the RPM, when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-β1 was enhanced during RPM culture for 24 h. Taking these results together, we suggest that chondrocytes exposed to the RPM seem to change their extracellular matrix production behaviour while they rearrange their cytoskeletal proteins prior to forming three-dimensional aggregates.

Originalsprog	Engelsk
Tidsskrift	PLOS ONE
Vol/bind	8
Nummer	11
Sider (fra-til)	e79057
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0079057
Status	Udgivet - 2013

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10.1371/journal.pone.0079057

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title = "Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine",

abstract = "Tissue engineering of chondrocytes on a Random Positioning Machine (RPM) is a new strategy for cartilage regeneration. Using a three-dimensional RPM, a device designed to simulate microgravity on Earth, we investigated the early effects of RPM exposure on human chondrocytes of six different donors after 30 min, 2 h, 4 h, 16 h, and 24 h and compared the results with the corresponding static controls cultured under normal gravity conditions. As little as 30 min of RPM exposure resulted in increased expression of several genes responsible for cell motility, structure and integrity (beta-actin); control of cell growth, cell proliferation, cell differentiation and apoptosis (TGF-β1, osteopontin); and cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours of RPM exposure disruptions in the vimentin network were detected. These changes were less dramatic after 16 hours on the RPM, when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-β1 was enhanced during RPM culture for 24 h. Taking these results together, we suggest that chondrocytes exposed to the RPM seem to change their extracellular matrix production behaviour while they rearrange their cytoskeletal proteins prior to forming three-dimensional aggregates.",

author = "Ganna Aleshcheva and Jayashree Sahana and Xiao Ma and Jens Hauslage and Ruth Hemmersbach and Marcel Egli and Manfred Infanger and Johann Bauer and Grimm, {Daniela Gabriele}",

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AU - Aleshcheva, Ganna

AU - Sahana, Jayashree

AU - Ma, Xiao

AU - Hauslage, Jens

AU - Hemmersbach, Ruth

AU - Egli, Marcel

AU - Infanger, Manfred

AU - Bauer, Johann

AU - Grimm, Daniela Gabriele

PY - 2013

Y1 - 2013

N2 - Tissue engineering of chondrocytes on a Random Positioning Machine (RPM) is a new strategy for cartilage regeneration. Using a three-dimensional RPM, a device designed to simulate microgravity on Earth, we investigated the early effects of RPM exposure on human chondrocytes of six different donors after 30 min, 2 h, 4 h, 16 h, and 24 h and compared the results with the corresponding static controls cultured under normal gravity conditions. As little as 30 min of RPM exposure resulted in increased expression of several genes responsible for cell motility, structure and integrity (beta-actin); control of cell growth, cell proliferation, cell differentiation and apoptosis (TGF-β1, osteopontin); and cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours of RPM exposure disruptions in the vimentin network were detected. These changes were less dramatic after 16 hours on the RPM, when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-β1 was enhanced during RPM culture for 24 h. Taking these results together, we suggest that chondrocytes exposed to the RPM seem to change their extracellular matrix production behaviour while they rearrange their cytoskeletal proteins prior to forming three-dimensional aggregates.

AB - Tissue engineering of chondrocytes on a Random Positioning Machine (RPM) is a new strategy for cartilage regeneration. Using a three-dimensional RPM, a device designed to simulate microgravity on Earth, we investigated the early effects of RPM exposure on human chondrocytes of six different donors after 30 min, 2 h, 4 h, 16 h, and 24 h and compared the results with the corresponding static controls cultured under normal gravity conditions. As little as 30 min of RPM exposure resulted in increased expression of several genes responsible for cell motility, structure and integrity (beta-actin); control of cell growth, cell proliferation, cell differentiation and apoptosis (TGF-β1, osteopontin); and cytoskeletal components such as microtubules (beta-tubulin) and intermediate filaments (vimentin). After 4 hours of RPM exposure disruptions in the vimentin network were detected. These changes were less dramatic after 16 hours on the RPM, when human chondrocytes appeared to reorganize their cytoskeleton. However, the gene expression and protein content of TGF-β1 was enhanced during RPM culture for 24 h. Taking these results together, we suggest that chondrocytes exposed to the RPM seem to change their extracellular matrix production behaviour while they rearrange their cytoskeletal proteins prior to forming three-dimensional aggregates.

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DO - 10.1371/journal.pone.0079057

M3 - Journal article

C2 - 24244418

SN - 1932-6203

VL - 8

SP - e79057

JO - PLOS ONE

JF - PLOS ONE

IS - 11

ER -

Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine

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