Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice

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Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice. / Zachariassen, Line Fisker; Krych, Lukasz; Rasmussen, Sara Hansborg; Nielsen, Dennis Sandris; Kot, Witold; Holm, Thomas Lindebo; Hansen, Axel Kornerup; Hansen, Camilla Hartmann Friis.

I: Journal of Immunology, Bind 202, Nr. 1, 01.01.2019, s. 142-150.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Zachariassen, LF, Krych, L, Rasmussen, SH, Nielsen, DS, Kot, W, Holm, TL, Hansen, AK & Hansen, CHF 2019, 'Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice', Journal of Immunology, bind 202, nr. 1, s. 142-150. https://doi.org/10.4049/jimmunol.1800666

APA

Zachariassen, L. F., Krych, L., Rasmussen, S. H., Nielsen, D. S., Kot, W., Holm, T. L., Hansen, A. K., & Hansen, C. H. F. (2019). Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice. Journal of Immunology, 202(1), 142-150. https://doi.org/10.4049/jimmunol.1800666

CBE

Zachariassen LF, Krych L, Rasmussen SH, Nielsen DS, Kot W, Holm TL, Hansen AK, Hansen CHF. 2019. Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice. Journal of Immunology. 202(1):142-150. https://doi.org/10.4049/jimmunol.1800666

MLA

Vancouver

Zachariassen LF, Krych L, Rasmussen SH, Nielsen DS, Kot W, Holm TL o.a. Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice. Journal of Immunology. 2019 jan 1;202(1):142-150. https://doi.org/10.4049/jimmunol.1800666

Author

Zachariassen, Line Fisker ; Krych, Lukasz ; Rasmussen, Sara Hansborg ; Nielsen, Dennis Sandris ; Kot, Witold ; Holm, Thomas Lindebo ; Hansen, Axel Kornerup ; Hansen, Camilla Hartmann Friis. / Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice. I: Journal of Immunology. 2019 ; Bind 202, Nr. 1. s. 142-150.

Bibtex

@article{689c12763aa54128bbde94a8b9e862d0,
title = "Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice",
abstract = "Epidemiological studies have shown that children born by cesarean section (CS) are at higher risk of developing chronic inflammatory diseases, and it has been suggested that a skewed gut microbial colonization process early in life and altered priming of the immune system are causative. The aim of this study was to clarify whether impaired regulatory immunity in CS-delivered C57BL/6 mice is dependent on gut microbiota (GM) disturbances. The GM of conventionally bred mice born by CS differed clearly from mice born by vaginal delivery. The proportion of regulatory T cells was reduced in mice born by CS, whereas the invariant NKT (iNKT) cell subset was increased compared with vaginal delivery mice. In addition, regulatory markers (Foxp3, Il10, Ctla4) and macrophage markers (Cd11c, Egr2, Nos2) were downregulated, whereas iNKT markers (Il4, Il15) were upregulated in ileum of CS-delivered mice. The GM of CS-delivered mice was sufficient to transfer the shifts in immunity associated with delivery mode when inoculated into germ-free mice. Feeding a prebiotic diet reestablished gene expression of intestinal immune markers and iNKT cells in CS mice but was not sufficient to restore the level of regulatory T cells. The results support that CS delivery is associated with microbiota-mediated shifts in regulatory immunity and, therefore, provide a basis for future microbiota-directed therapeutics to infants born by CS.",
keywords = "FECAL MICROBIOTA, EARLY-LIFE, T-CELLS, DELIVERY, INFANTS, MODE, RISK, HOMEOSTASIS, SHAPES, IMPACT",
author = "Zachariassen, {Line Fisker} and Lukasz Krych and Rasmussen, {Sara Hansborg} and Nielsen, {Dennis Sandris} and Witold Kot and Holm, {Thomas Lindebo} and Hansen, {Axel Kornerup} and Hansen, {Camilla Hartmann Friis}",
year = "2019",
month = jan,
day = "1",
doi = "10.4049/jimmunol.1800666",
language = "English",
volume = "202",
pages = "142--150",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

RIS

TY - JOUR

T1 - Cesarean Section Induces Microbiota-Regulated Immune Disturbances in C57BL/6 Mice

AU - Zachariassen, Line Fisker

AU - Krych, Lukasz

AU - Rasmussen, Sara Hansborg

AU - Nielsen, Dennis Sandris

AU - Kot, Witold

AU - Holm, Thomas Lindebo

AU - Hansen, Axel Kornerup

AU - Hansen, Camilla Hartmann Friis

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Epidemiological studies have shown that children born by cesarean section (CS) are at higher risk of developing chronic inflammatory diseases, and it has been suggested that a skewed gut microbial colonization process early in life and altered priming of the immune system are causative. The aim of this study was to clarify whether impaired regulatory immunity in CS-delivered C57BL/6 mice is dependent on gut microbiota (GM) disturbances. The GM of conventionally bred mice born by CS differed clearly from mice born by vaginal delivery. The proportion of regulatory T cells was reduced in mice born by CS, whereas the invariant NKT (iNKT) cell subset was increased compared with vaginal delivery mice. In addition, regulatory markers (Foxp3, Il10, Ctla4) and macrophage markers (Cd11c, Egr2, Nos2) were downregulated, whereas iNKT markers (Il4, Il15) were upregulated in ileum of CS-delivered mice. The GM of CS-delivered mice was sufficient to transfer the shifts in immunity associated with delivery mode when inoculated into germ-free mice. Feeding a prebiotic diet reestablished gene expression of intestinal immune markers and iNKT cells in CS mice but was not sufficient to restore the level of regulatory T cells. The results support that CS delivery is associated with microbiota-mediated shifts in regulatory immunity and, therefore, provide a basis for future microbiota-directed therapeutics to infants born by CS.

AB - Epidemiological studies have shown that children born by cesarean section (CS) are at higher risk of developing chronic inflammatory diseases, and it has been suggested that a skewed gut microbial colonization process early in life and altered priming of the immune system are causative. The aim of this study was to clarify whether impaired regulatory immunity in CS-delivered C57BL/6 mice is dependent on gut microbiota (GM) disturbances. The GM of conventionally bred mice born by CS differed clearly from mice born by vaginal delivery. The proportion of regulatory T cells was reduced in mice born by CS, whereas the invariant NKT (iNKT) cell subset was increased compared with vaginal delivery mice. In addition, regulatory markers (Foxp3, Il10, Ctla4) and macrophage markers (Cd11c, Egr2, Nos2) were downregulated, whereas iNKT markers (Il4, Il15) were upregulated in ileum of CS-delivered mice. The GM of CS-delivered mice was sufficient to transfer the shifts in immunity associated with delivery mode when inoculated into germ-free mice. Feeding a prebiotic diet reestablished gene expression of intestinal immune markers and iNKT cells in CS mice but was not sufficient to restore the level of regulatory T cells. The results support that CS delivery is associated with microbiota-mediated shifts in regulatory immunity and, therefore, provide a basis for future microbiota-directed therapeutics to infants born by CS.

KW - FECAL MICROBIOTA

KW - EARLY-LIFE

KW - T-CELLS

KW - DELIVERY

KW - INFANTS

KW - MODE

KW - RISK

KW - HOMEOSTASIS

KW - SHAPES

KW - IMPACT

U2 - 10.4049/jimmunol.1800666

DO - 10.4049/jimmunol.1800666

M3 - Journal article

C2 - 30487172

VL - 202

SP - 142

EP - 150

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -