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CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis

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CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis. / Yano, Koji; Shibata, Satoshi; Chen, Wen-Li; Sato, Shusei; Kaneko, Takakazu; Jurkiewicz, Anna; Sandal, Niels; Banba, Mari; Imaizumi-Anraku, Haruko; Kojima, Tomoko; Ohtomo, Ryo; Szczyglowski, Krzysztof; Stougaard, Jens; Tabata, Satoshi; Hayashi, Makoto; Kouchi, Hiroshi; Umehara, Yosuke.

I: Plant Journal, Bind 60, Nr. 1, 2009, s. 168-180.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Yano, K, Shibata, S, Chen, W-L, Sato, S, Kaneko, T, Jurkiewicz, A, Sandal, N, Banba, M, Imaizumi-Anraku, H, Kojima, T, Ohtomo, R, Szczyglowski, K, Stougaard, J, Tabata, S, Hayashi, M, Kouchi, H & Umehara, Y 2009, 'CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis', Plant Journal, bind 60, nr. 1, s. 168-180. https://doi.org/10.1111/j.1365-313X.2009.03943.x

APA

CBE

Yano K, Shibata S, Chen W-L, Sato S, Kaneko T, Jurkiewicz A, Sandal N, Banba M, Imaizumi-Anraku H, Kojima T, Ohtomo R, Szczyglowski K, Stougaard J, Tabata S, Hayashi M, Kouchi H, Umehara Y. 2009. CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis. Plant Journal. 60(1):168-180. https://doi.org/10.1111/j.1365-313X.2009.03943.x

MLA

Vancouver

Author

Yano, Koji ; Shibata, Satoshi ; Chen, Wen-Li ; Sato, Shusei ; Kaneko, Takakazu ; Jurkiewicz, Anna ; Sandal, Niels ; Banba, Mari ; Imaizumi-Anraku, Haruko ; Kojima, Tomoko ; Ohtomo, Ryo ; Szczyglowski, Krzysztof ; Stougaard, Jens ; Tabata, Satoshi ; Hayashi, Makoto ; Kouchi, Hiroshi ; Umehara, Yosuke. / CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis. I: Plant Journal. 2009 ; Bind 60, Nr. 1. s. 168-180.

Bibtex

@article{47c4b4f0a1e511dea092000ea68e967b,
title = "CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis",
abstract = "Endosymbiotic infection of legume plants by Rhizobium bacteria is initiated through infection threads (ITs) which are initiated within and penetrate from root hairs and deliver the endosymbionts into nodule cells. Despite recent progress in understanding the mutual recognition and early symbiotic signaling cascades in host legumes, the molecular mechanisms underlying bacterial infection processes and successive nodule organogenesis are still poorly understood. We isolated a novel symbiotic mutant of Lotus japonicus, cerberus, which shows defects in IT formation and nodule organogenesis. Map-based cloning of the causal gene allowed us to identify the CERBERUS gene, which encodes a novel protein containing a U-box domain and WD-40 repeats. CERBERUS expression was detected in the roots and nodules, and was enhanced after inoculation of Mesorhizobium loti. Strong expression was detected in developing nodule primordia and the infected zone of mature nodules. In cerberus mutants, Rhizobium colonized curled root hair tips, but hardly penetrated into root hair cells. The occasional ITs that were formed inside the root hair cells were mostly arrested within the epidermal cell layer. Nodule organogenesis was aborted prematurely, resulting in the formation of a large number of small bumps which contained no endosymbiotic bacteria. These phenotypic and genetic analyses, together with comparisons with other legume mutants with defects in IT formation, indicate that CERBERUS plays a critical role in the very early steps of IT formation as well as in growth and differentiation of nodules.",
author = "Koji Yano and Satoshi Shibata and Wen-Li Chen and Shusei Sato and Takakazu Kaneko and Anna Jurkiewicz and Niels Sandal and Mari Banba and Haruko Imaizumi-Anraku and Tomoko Kojima and Ryo Ohtomo and Krzysztof Szczyglowski and Jens Stougaard and Satoshi Tabata and Makoto Hayashi and Hiroshi Kouchi and Yosuke Umehara",
year = "2009",
doi = "10.1111/j.1365-313X.2009.03943.x",
language = "English",
volume = "60",
pages = "168--180",
journal = "The Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - CERBERUS, a novel U-box protein containing WD-40 repeats, is required for formation of the infection thread and nodule development in the legume-Rhizobium symbiosis

AU - Yano, Koji

AU - Shibata, Satoshi

AU - Chen, Wen-Li

AU - Sato, Shusei

AU - Kaneko, Takakazu

AU - Jurkiewicz, Anna

AU - Sandal, Niels

AU - Banba, Mari

AU - Imaizumi-Anraku, Haruko

AU - Kojima, Tomoko

AU - Ohtomo, Ryo

AU - Szczyglowski, Krzysztof

AU - Stougaard, Jens

AU - Tabata, Satoshi

AU - Hayashi, Makoto

AU - Kouchi, Hiroshi

AU - Umehara, Yosuke

PY - 2009

Y1 - 2009

N2 - Endosymbiotic infection of legume plants by Rhizobium bacteria is initiated through infection threads (ITs) which are initiated within and penetrate from root hairs and deliver the endosymbionts into nodule cells. Despite recent progress in understanding the mutual recognition and early symbiotic signaling cascades in host legumes, the molecular mechanisms underlying bacterial infection processes and successive nodule organogenesis are still poorly understood. We isolated a novel symbiotic mutant of Lotus japonicus, cerberus, which shows defects in IT formation and nodule organogenesis. Map-based cloning of the causal gene allowed us to identify the CERBERUS gene, which encodes a novel protein containing a U-box domain and WD-40 repeats. CERBERUS expression was detected in the roots and nodules, and was enhanced after inoculation of Mesorhizobium loti. Strong expression was detected in developing nodule primordia and the infected zone of mature nodules. In cerberus mutants, Rhizobium colonized curled root hair tips, but hardly penetrated into root hair cells. The occasional ITs that were formed inside the root hair cells were mostly arrested within the epidermal cell layer. Nodule organogenesis was aborted prematurely, resulting in the formation of a large number of small bumps which contained no endosymbiotic bacteria. These phenotypic and genetic analyses, together with comparisons with other legume mutants with defects in IT formation, indicate that CERBERUS plays a critical role in the very early steps of IT formation as well as in growth and differentiation of nodules.

AB - Endosymbiotic infection of legume plants by Rhizobium bacteria is initiated through infection threads (ITs) which are initiated within and penetrate from root hairs and deliver the endosymbionts into nodule cells. Despite recent progress in understanding the mutual recognition and early symbiotic signaling cascades in host legumes, the molecular mechanisms underlying bacterial infection processes and successive nodule organogenesis are still poorly understood. We isolated a novel symbiotic mutant of Lotus japonicus, cerberus, which shows defects in IT formation and nodule organogenesis. Map-based cloning of the causal gene allowed us to identify the CERBERUS gene, which encodes a novel protein containing a U-box domain and WD-40 repeats. CERBERUS expression was detected in the roots and nodules, and was enhanced after inoculation of Mesorhizobium loti. Strong expression was detected in developing nodule primordia and the infected zone of mature nodules. In cerberus mutants, Rhizobium colonized curled root hair tips, but hardly penetrated into root hair cells. The occasional ITs that were formed inside the root hair cells were mostly arrested within the epidermal cell layer. Nodule organogenesis was aborted prematurely, resulting in the formation of a large number of small bumps which contained no endosymbiotic bacteria. These phenotypic and genetic analyses, together with comparisons with other legume mutants with defects in IT formation, indicate that CERBERUS plays a critical role in the very early steps of IT formation as well as in growth and differentiation of nodules.

U2 - 10.1111/j.1365-313X.2009.03943.x

DO - 10.1111/j.1365-313X.2009.03943.x

M3 - Journal article

C2 - 19508425

VL - 60

SP - 168

EP - 180

JO - The Plant Journal

JF - The Plant Journal

SN - 0960-7412

IS - 1

ER -