CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth

Michael Taleski; Kelly Chapman; Ondřej Novák; Thomas Schmülling; Manuel Frank; Michael A Djordjevic

doi:10.1038/s41467-023-37282-6

CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth

Michael Taleski
, Kelly Chapman
, Ondřej Novák
, Thomas Schmülling
, Manuel Frank^*
, Michael A Djordjevic^*

^*Corresponding author af dette arbejde

Institut for Molekylærbiologi og Genetik - Plantemolekylærbiologi

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

26 Citationer (Scopus)

Abstract

C-TERMINALLY ENCODED PEPTIDE (CEP) and cytokinin hormones act over short and long distances to control plant responses to environmental cues. CEP and cytokinin pathway mutants share phenotypes, however, it is not known if these pathways intersect. We show that CEP and cytokinin signalling converge on CEP DOWNSTREAM (CEPD) glutaredoxins to inhibit primary root growth. CEP inhibition of root growth was impaired in mutants defective in trans-zeatin (tZ)-type cytokinin biosynthesis, transport, perception, and output. Concordantly, mutants affected in CEP RECEPTOR 1 showed reduced root growth inhibition in response to tZ, and altered levels of tZ-type cytokinins. Grafting and organ-specific hormone treatments showed that tZ-mediated root growth inhibition involved CEPD activity in roots. By contrast, root growth inhibition by CEP depended on shoot CEPD function. The results demonstrate that CEP and cytokinin pathways intersect, and utilise signalling circuits in separate organs involving common glutaredoxin genes to coordinate root growth.

Originalsprog	Engelsk
Artikelnummer	1683
Tidsskrift	Nature Communications
Vol/bind	14
Nummer	1
Antal sider	11
ISSN	2041-1723
DOI	https://doi.org/10.1038/s41467-023-37282-6
Status	Udgivet - dec. 2023

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title = "CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth",

abstract = "C-TERMINALLY ENCODED PEPTIDE (CEP) and cytokinin hormones act over short and long distances to control plant responses to environmental cues. CEP and cytokinin pathway mutants share phenotypes, however, it is not known if these pathways intersect. We show that CEP and cytokinin signalling converge on CEP DOWNSTREAM (CEPD) glutaredoxins to inhibit primary root growth. CEP inhibition of root growth was impaired in mutants defective in trans-zeatin (tZ)-type cytokinin biosynthesis, transport, perception, and output. Concordantly, mutants affected in CEP RECEPTOR 1 showed reduced root growth inhibition in response to tZ, and altered levels of tZ-type cytokinins. Grafting and organ-specific hormone treatments showed that tZ-mediated root growth inhibition involved CEPD activity in roots. By contrast, root growth inhibition by CEP depended on shoot CEPD function. The results demonstrate that CEP and cytokinin pathways intersect, and utilise signalling circuits in separate organs involving common glutaredoxin genes to coordinate root growth.",

author = "Michael Taleski and Kelly Chapman and Ond{\v r}ej Nov{\'a}k and Thomas Schm{\"u}lling and Manuel Frank and Djordjevic, \{Michael A\}",

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T1 - CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth

AU - Taleski, Michael

AU - Chapman, Kelly

AU - Novák, Ondřej

AU - Schmülling, Thomas

AU - Frank, Manuel

AU - Djordjevic, Michael A

PY - 2023/12

Y1 - 2023/12

N2 - C-TERMINALLY ENCODED PEPTIDE (CEP) and cytokinin hormones act over short and long distances to control plant responses to environmental cues. CEP and cytokinin pathway mutants share phenotypes, however, it is not known if these pathways intersect. We show that CEP and cytokinin signalling converge on CEP DOWNSTREAM (CEPD) glutaredoxins to inhibit primary root growth. CEP inhibition of root growth was impaired in mutants defective in trans-zeatin (tZ)-type cytokinin biosynthesis, transport, perception, and output. Concordantly, mutants affected in CEP RECEPTOR 1 showed reduced root growth inhibition in response to tZ, and altered levels of tZ-type cytokinins. Grafting and organ-specific hormone treatments showed that tZ-mediated root growth inhibition involved CEPD activity in roots. By contrast, root growth inhibition by CEP depended on shoot CEPD function. The results demonstrate that CEP and cytokinin pathways intersect, and utilise signalling circuits in separate organs involving common glutaredoxin genes to coordinate root growth.

AB - C-TERMINALLY ENCODED PEPTIDE (CEP) and cytokinin hormones act over short and long distances to control plant responses to environmental cues. CEP and cytokinin pathway mutants share phenotypes, however, it is not known if these pathways intersect. We show that CEP and cytokinin signalling converge on CEP DOWNSTREAM (CEPD) glutaredoxins to inhibit primary root growth. CEP inhibition of root growth was impaired in mutants defective in trans-zeatin (tZ)-type cytokinin biosynthesis, transport, perception, and output. Concordantly, mutants affected in CEP RECEPTOR 1 showed reduced root growth inhibition in response to tZ, and altered levels of tZ-type cytokinins. Grafting and organ-specific hormone treatments showed that tZ-mediated root growth inhibition involved CEPD activity in roots. By contrast, root growth inhibition by CEP depended on shoot CEPD function. The results demonstrate that CEP and cytokinin pathways intersect, and utilise signalling circuits in separate organs involving common glutaredoxin genes to coordinate root growth.

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DO - 10.1038/s41467-023-37282-6

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SN - 2041-1723

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JO - Nature Communications

JF - Nature Communications

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ER -

CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth

Abstract

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