Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries

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Standard

Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries. / Raniolo, Sofia; Croce, Stefano; Thomsen, Rasmus P.; Okholm, Anders H.; Unida, Valeria; Iacovelli, Federico; Manetto, Antonio; Kjems, Jørgen; Desideri, Alessandro; Biocca, Silvia.

I: Nanoscale, Bind 11, Nr. 22, 06.2019, s. 10808-10818.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Raniolo, S, Croce, S, Thomsen, RP, Okholm, AH, Unida, V, Iacovelli, F, Manetto, A, Kjems, J, Desideri, A & Biocca, S 2019, 'Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries', Nanoscale, bind 11, nr. 22, s. 10808-10818. https://doi.org/10.1039/c9nr02006c

APA

Raniolo, S., Croce, S., Thomsen, R. P., Okholm, A. H., Unida, V., Iacovelli, F., Manetto, A., Kjems, J., Desideri, A., & Biocca, S. (2019). Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries. Nanoscale, 11(22), 10808-10818. https://doi.org/10.1039/c9nr02006c

CBE

Raniolo S, Croce S, Thomsen RP, Okholm AH, Unida V, Iacovelli F, Manetto A, Kjems J, Desideri A, Biocca S. 2019. Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries. Nanoscale. 11(22):10808-10818. https://doi.org/10.1039/c9nr02006c

MLA

Vancouver

Raniolo S, Croce S, Thomsen RP, Okholm AH, Unida V, Iacovelli F o.a. Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries. Nanoscale. 2019 jun;11(22):10808-10818. https://doi.org/10.1039/c9nr02006c

Author

Raniolo, Sofia ; Croce, Stefano ; Thomsen, Rasmus P. ; Okholm, Anders H. ; Unida, Valeria ; Iacovelli, Federico ; Manetto, Antonio ; Kjems, Jørgen ; Desideri, Alessandro ; Biocca, Silvia. / Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries. I: Nanoscale. 2019 ; Bind 11, Nr. 22. s. 10808-10818.

Bibtex

@article{54c64f23ddf64c2aa380e8b06be6a416,
title = "Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries",
abstract = "DNA nanostructures with different sizes and shapes, assembled through either covalent or non-covalent bonds, namely tetrahedral and octahedral nanocages, rod-shaped chainmails, square box and rectangular DNA origami structures, were compared for their stability in serum, cell surface binding, internalization efficiency, and intracellular degradation rate. For cell internalization a specific cell system, highly expressing the scavenger receptor LOX-1 was used. The results indicate that LOX-1 binds and internalizes a broad family of DNA structures of different sizes that, however, have a different fate and lifetime inside the cells. Covalently linked tetrahedra, octahedra or chainmails are intact inside cells for up to 18 hours whilst the same DNA nanostructures without covalent bonds along with square box and rectangular origami are rapidly degraded. These data suggest that non-covalently linked structures may be useful for fast drug release whilst the covalently-linked structures could be appropriate vehicles for slow release of molecules.",
author = "Sofia Raniolo and Stefano Croce and Thomsen, {Rasmus P.} and Okholm, {Anders H.} and Valeria Unida and Federico Iacovelli and Antonio Manetto and J{\o}rgen Kjems and Alessandro Desideri and Silvia Biocca",
year = "2019",
month = jun,
doi = "10.1039/c9nr02006c",
language = "English",
volume = "11",
pages = "10808--10818",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "ROYAL SOC CHEMISTRY",
number = "22",

}

RIS

TY - JOUR

T1 - Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries

AU - Raniolo, Sofia

AU - Croce, Stefano

AU - Thomsen, Rasmus P.

AU - Okholm, Anders H.

AU - Unida, Valeria

AU - Iacovelli, Federico

AU - Manetto, Antonio

AU - Kjems, Jørgen

AU - Desideri, Alessandro

AU - Biocca, Silvia

PY - 2019/6

Y1 - 2019/6

N2 - DNA nanostructures with different sizes and shapes, assembled through either covalent or non-covalent bonds, namely tetrahedral and octahedral nanocages, rod-shaped chainmails, square box and rectangular DNA origami structures, were compared for their stability in serum, cell surface binding, internalization efficiency, and intracellular degradation rate. For cell internalization a specific cell system, highly expressing the scavenger receptor LOX-1 was used. The results indicate that LOX-1 binds and internalizes a broad family of DNA structures of different sizes that, however, have a different fate and lifetime inside the cells. Covalently linked tetrahedra, octahedra or chainmails are intact inside cells for up to 18 hours whilst the same DNA nanostructures without covalent bonds along with square box and rectangular origami are rapidly degraded. These data suggest that non-covalently linked structures may be useful for fast drug release whilst the covalently-linked structures could be appropriate vehicles for slow release of molecules.

AB - DNA nanostructures with different sizes and shapes, assembled through either covalent or non-covalent bonds, namely tetrahedral and octahedral nanocages, rod-shaped chainmails, square box and rectangular DNA origami structures, were compared for their stability in serum, cell surface binding, internalization efficiency, and intracellular degradation rate. For cell internalization a specific cell system, highly expressing the scavenger receptor LOX-1 was used. The results indicate that LOX-1 binds and internalizes a broad family of DNA structures of different sizes that, however, have a different fate and lifetime inside the cells. Covalently linked tetrahedra, octahedra or chainmails are intact inside cells for up to 18 hours whilst the same DNA nanostructures without covalent bonds along with square box and rectangular origami are rapidly degraded. These data suggest that non-covalently linked structures may be useful for fast drug release whilst the covalently-linked structures could be appropriate vehicles for slow release of molecules.

UR - http://www.scopus.com/inward/record.url?scp=85067057635&partnerID=8YFLogxK

U2 - 10.1039/c9nr02006c

DO - 10.1039/c9nr02006c

M3 - Journal article

C2 - 31134260

AN - SCOPUS:85067057635

VL - 11

SP - 10808

EP - 10818

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 22

ER -