Cellular milk production: Proteins and minerals in secretomes from cultivated bovine milk-derived mammary cells

TY - JOUR

T1 - Cellular milk production: Proteins and minerals in secretomes from cultivated bovine milk-derived mammary cells

AU - Che, Jing

AU - Yue, Yuan

AU - Sirinayake Lokuge, Gayani Madushani

AU - Nielsen, Søren Drud-Heydary

AU - Sundekilde, Ulrik Kræmer

AU - Purup, Stig

AU - Larsen, Lotte Bach

AU - Poulsen, Nina Aagaard

PY - 2025/3/1

Y1 - 2025/3/1

N2 - This study explores the feasibility of utilizing in vitro cultivated milk-derived bovine mammary epithelial cells (bMECs) for the production of milk constituents. BMECs were isolated from milk and treated with various lactogenic agents in 3D transwell systems. By proteomics, >900 proteins were identified and quantified in the secretomes, including >100 milk-related proteins such as caseins and enzymes. Despite limited secretion of total proteins and major milk proteins, 110 proteins were found phosphorylated, including 27 involved in metal- or calcium-binding. Mineral analysis confirmed that 6–9 % of minerals in secretomes were associated with proteins. Notably, six proteins, including prolactin, were secreted into the basolateral side of bMECs without lactogenic treatment, suggesting their local de novo synthesis. This research advances our understanding of bMECs biology, as well as the compositional and functional features of their secretomes, highlighting their potential for sustainable production of functional milk proteins, meanwhile emphasizing the need for further optimization.

AB - This study explores the feasibility of utilizing in vitro cultivated milk-derived bovine mammary epithelial cells (bMECs) for the production of milk constituents. BMECs were isolated from milk and treated with various lactogenic agents in 3D transwell systems. By proteomics, >900 proteins were identified and quantified in the secretomes, including >100 milk-related proteins such as caseins and enzymes. Despite limited secretion of total proteins and major milk proteins, 110 proteins were found phosphorylated, including 27 involved in metal- or calcium-binding. Mineral analysis confirmed that 6–9 % of minerals in secretomes were associated with proteins. Notably, six proteins, including prolactin, were secreted into the basolateral side of bMECs without lactogenic treatment, suggesting their local de novo synthesis. This research advances our understanding of bMECs biology, as well as the compositional and functional features of their secretomes, highlighting their potential for sustainable production of functional milk proteins, meanwhile emphasizing the need for further optimization.

KW - Cellular agriculture

KW - Mammary epithelial cell

KW - Milk production

KW - Protein-associated mineral

KW - Proteomics

KW - Secretomes

UR - http://www.scopus.com/inward/record.url?scp=85211214642&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2024.142386

DO - 10.1016/j.foodchem.2024.142386

M3 - Journal article

C2 - 39657482

SN - 0308-8146

VL - 467

JO - Food Chemistry

JF - Food Chemistry

M1 - 142386

ER -