TY - JOUR
T1 - Cell Cycle Modulation through Subcellular Spatially Resolved Production of Singlet Oxygen via Direct 765 nm Irradiation: Manipulating the Onset of Mitosis.
AU - Blazquez-Castro, Alfonso
AU - Breitenbach, Thomas
AU - Ogilby, Peter Remsen
PY - 2018
Y1 - 2018
N2 - Reactive oxygen species, ROS, are acknowledged signaling molecules in cellular processes. Singlet molecular oxygen, O
2(a
1Δ
g), is one ROS that can initiate cell responses that range from death to proliferation. To better understand the mechanisms involved, it is necessary to further investigate cell response to the “dose” of O
2(a
1Δ
g) that has been selectively produced at the expense of other ROS. In this context, dose refers not just to the amount of O
2(a
1Δ
g) produced, but also to the subcellular spatial domain in which it is produced. In this study, we selectively produced small and non-toxic amounts of O
2(a
1Δ
g) in sensitizer-free experiments by irradiating oxygen at 765 nm using a laser focused either into the nucleus or cytoplasm of HeLa cells. We find that O
2(a
1Δ
g)-mediated cell proliferation depends appreciably on the site of O
2(a
1Δ
g) production. At the same incident laser power, irradiation into the cytoplasm elicits moderate enhancement of proliferation, whereas irradiation into the nucleus leads to an appreciable delay in the onset and completion of mitosis. We discuss these results in light of what is known about the intracellular photophysics of O
2(a
1Δ
g) and the redox state of different cell domains.
AB - Reactive oxygen species, ROS, are acknowledged signaling molecules in cellular processes. Singlet molecular oxygen, O
2(a
1Δ
g), is one ROS that can initiate cell responses that range from death to proliferation. To better understand the mechanisms involved, it is necessary to further investigate cell response to the “dose” of O
2(a
1Δ
g) that has been selectively produced at the expense of other ROS. In this context, dose refers not just to the amount of O
2(a
1Δ
g) produced, but also to the subcellular spatial domain in which it is produced. In this study, we selectively produced small and non-toxic amounts of O
2(a
1Δ
g) in sensitizer-free experiments by irradiating oxygen at 765 nm using a laser focused either into the nucleus or cytoplasm of HeLa cells. We find that O
2(a
1Δ
g)-mediated cell proliferation depends appreciably on the site of O
2(a
1Δ
g) production. At the same incident laser power, irradiation into the cytoplasm elicits moderate enhancement of proliferation, whereas irradiation into the nucleus leads to an appreciable delay in the onset and completion of mitosis. We discuss these results in light of what is known about the intracellular photophysics of O
2(a
1Δ
g) and the redox state of different cell domains.
UR - http://www.scopus.com/inward/record.url?scp=85054772846&partnerID=8YFLogxK
U2 - 10.1039/c8pp00338f
DO - 10.1039/c8pp00338f
M3 - Journal article
C2 - 30095847
SN - 1474-905X
VL - 17
SP - 1310
EP - 1318
JO - Photochemical & Photobiological Sciences
JF - Photochemical & Photobiological Sciences
IS - 10
ER -