Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis

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DOI

  • Thomas Hackenberg
  • ,
  • Trine Juul
  • ,
  • Aija Auzina
  • ,
  • Sonia Gwizdz, Department of Molecular Biology and Genetics, Aarhus University, Danmark
  • Anna Malolepszy, Danmark
  • Katrien Van Der Kelen, VIB Department of Plant Systems Biology, Ghent University, Belgien
  • Svend Dam, Danmark
  • Simon Bressendorff, Københavns Universitet, Danmark
  • Andrea Maria Lorentzen, eDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Danmark
  • Peter Roepstorff, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Danmark
  • Kåre Lehmann Nielsen, Aalborg Universitet, Danmark
  • Jan-Elo Jørgensen
  • Daniel Hofius, Københavns Universitet, Danmark
  • Frank Van Breusegem, VIB Department of Plant Systems Biology, Ghent University, Belgien
  • Morten Petersen, Københavns Universitet, Danmark
  • Stig Uggerhøj Andersen
Programmed cell death often depends on generation of reactive oxygen species, which can be detoxified by antioxidative enzymes, including catalases. We previously isolated catalase-deficient mutants (cat2) in a screen for resistance to hydroxyurea-induced cell death. Here, we identify an Arabidopsis thaliana hydroxyurea-resistant autophagy mutant, atg2, which also shows reduced sensitivity to cell death triggered by the bacterial effector avrRpm1. To test if catalase deficiency likewise affected both hydroxyurea and avrRpm1 sensitivity, we selected mutants with extremely low catalase activities and showed that they carried mutations in a gene that we named NO CATALASE ACTIVITY1 (NCA1). nca1 mutants showed severely reduced activities of all three catalase isoforms in Arabidopsis, and loss of NCA1 function led to strong suppression of RPM1-triggered cell death. Basal and starvation-induced autophagy appeared normal in the nca1 and cat2 mutants. By contrast, autophagic degradation induced by avrRpm1 challenge was compromised, indicating that catalase acted upstream of immunity-triggered autophagy. The direct interaction of catalase with reactive oxygen species could allow catalase to act as a molecular link between reactive oxygen species and the promotion of autophagy-dependent cell death.
OriginalsprogEngelsk
TidsskriftPlant Cell
Vol/bind25
Nummer11
Sider (fra-til)4616-4626
Antal sider11
ISSN1040-4651
DOI
StatusUdgivet - 27 nov. 2013

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