Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during diving

Naim M. Bautista; Christian Damsgaard; Angela Fago; Tobias Wang

doi:10.1242/jeb.242435

Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during diving

Naim M. Bautista, Christian Damsgaard^*, Angela Fago, Tobias Wang

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

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Abstract

The ability of crocodilian haemoglobins to bind HCO3- has been appreciated for more than half a century, but the functional implication of this exceptional mechanism has not previously been assessed in vivo. Therefore, the goal of the present study was to address the hypothesis that CO2 primarily binds to haemoglobin, rather than being accumulated in plasma as in other vertebrates, during diving in caimans. Here, we demonstrate that CO2 primarily accumulates within the erythrocyte during diving and that most of the accumulated CO2 is bound to haemoglobin. Furthermore, we show that this HCO3- binding is tightly associated with the progressive blood deoxygenation during diving; therefore, crocodilians differ from the classic vertebrate pattern, where HCO3- accumulates in the plasma upon excretion from the erythrocytes by the Cl-/HCO3- exchanger.

Originalsprog	Engelsk
Artikelnummer	242435
Tidsskrift	Journal of Experimental Biology
Vol/bind	224
Nummer	9
Antal sider	6
ISSN	0022-0949
DOI	https://doi.org/10.1242/jeb.242435
Status	Udgivet - maj 2021

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10.1242/jeb.242435

Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during divingForlagets udgivne version, 874 KBLicens: Andet

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title = "Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during diving",

abstract = "The ability of crocodilian haemoglobins to bind HCO3- has been appreciated for more than half a century, but the functional implication of this exceptional mechanism has not previously been assessed in vivo. Therefore, the goal of the present study was to address the hypothesis that CO2 primarily binds to haemoglobin, rather than being accumulated in plasma as in other vertebrates, during diving in caimans. Here, we demonstrate that CO2 primarily accumulates within the erythrocyte during diving and that most of the accumulated CO2 is bound to haemoglobin. Furthermore, we show that this HCO3- binding is tightly associated with the progressive blood deoxygenation during diving; therefore, crocodilians differ from the classic vertebrate pattern, where HCO3- accumulates in the plasma upon excretion from the erythrocytes by the Cl-/HCO3- exchanger.",

keywords = "ALLIGATOR BLOOD, ALLOSTERIC REGULATION, Blood gases, Blood-oxygen affinity, CROCODILE, CROCODYLUS-POROSUS, Haemoglobin-bicarbonate binding, OXYGEN-BINDING, Reptile, TRANSPORT, pH",

author = "Bautista, {Naim M.} and Christian Damsgaard and Angela Fago and Tobias Wang",

year = "2021",

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journal = "Journal of Experimental Biology",

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T1 - Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during diving

AU - Bautista, Naim M.

AU - Damsgaard, Christian

AU - Fago, Angela

AU - Wang, Tobias

PY - 2021/5

Y1 - 2021/5

N2 - The ability of crocodilian haemoglobins to bind HCO3- has been appreciated for more than half a century, but the functional implication of this exceptional mechanism has not previously been assessed in vivo. Therefore, the goal of the present study was to address the hypothesis that CO2 primarily binds to haemoglobin, rather than being accumulated in plasma as in other vertebrates, during diving in caimans. Here, we demonstrate that CO2 primarily accumulates within the erythrocyte during diving and that most of the accumulated CO2 is bound to haemoglobin. Furthermore, we show that this HCO3- binding is tightly associated with the progressive blood deoxygenation during diving; therefore, crocodilians differ from the classic vertebrate pattern, where HCO3- accumulates in the plasma upon excretion from the erythrocytes by the Cl-/HCO3- exchanger.

AB - The ability of crocodilian haemoglobins to bind HCO3- has been appreciated for more than half a century, but the functional implication of this exceptional mechanism has not previously been assessed in vivo. Therefore, the goal of the present study was to address the hypothesis that CO2 primarily binds to haemoglobin, rather than being accumulated in plasma as in other vertebrates, during diving in caimans. Here, we demonstrate that CO2 primarily accumulates within the erythrocyte during diving and that most of the accumulated CO2 is bound to haemoglobin. Furthermore, we show that this HCO3- binding is tightly associated with the progressive blood deoxygenation during diving; therefore, crocodilians differ from the classic vertebrate pattern, where HCO3- accumulates in the plasma upon excretion from the erythrocytes by the Cl-/HCO3- exchanger.

KW - ALLIGATOR BLOOD

KW - ALLOSTERIC REGULATION

KW - Blood gases

KW - Blood-oxygen affinity

KW - CROCODILE

KW - CROCODYLUS-POROSUS

KW - Haemoglobin-bicarbonate binding

KW - OXYGEN-BINDING

KW - Reptile

KW - TRANSPORT

KW - pH

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U2 - 10.1242/jeb.242435

DO - 10.1242/jeb.242435

M3 - Journal article

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SN - 0022-0949

VL - 224

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

IS - 9

M1 - 242435

ER -

Carbon dioxide and bicarbonate accumulation in caiman erythrocytes during diving

Abstract

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