Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry

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Standard

Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. / Månsson, Viktor; Gilsdorf, Janet R.; Kahlmeter, Gunnar; Kilian, Mogens; Kroll, J. Simon; Riesbeck, Kristian; Resman, Fredrik.

I: Emerging Infectious Diseases , Bind 24, Nr. 3, 01.03.2018, s. 443-452.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Månsson, V, Gilsdorf, JR, Kahlmeter, G, Kilian, M, Kroll, JS, Riesbeck, K & Resman, F 2018, 'Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry', Emerging Infectious Diseases , bind 24, nr. 3, s. 443-452. https://doi.org/10.3201/eid2403.170459

APA

Månsson, V., Gilsdorf, J. R., Kahlmeter, G., Kilian, M., Kroll, J. S., Riesbeck, K., & Resman, F. (2018). Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. Emerging Infectious Diseases , 24(3), 443-452. https://doi.org/10.3201/eid2403.170459

CBE

Månsson V, Gilsdorf JR, Kahlmeter G, Kilian M, Kroll JS, Riesbeck K, Resman F. 2018. Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. Emerging Infectious Diseases . 24(3):443-452. https://doi.org/10.3201/eid2403.170459

MLA

Vancouver

Månsson V, Gilsdorf JR, Kahlmeter G, Kilian M, Kroll JS, Riesbeck K o.a. Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. Emerging Infectious Diseases . 2018 mar 1;24(3):443-452. https://doi.org/10.3201/eid2403.170459

Author

Månsson, Viktor ; Gilsdorf, Janet R. ; Kahlmeter, Gunnar ; Kilian, Mogens ; Kroll, J. Simon ; Riesbeck, Kristian ; Resman, Fredrik. / Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. I: Emerging Infectious Diseases . 2018 ; Bind 24, Nr. 3. s. 443-452.

Bibtex

@article{fb9077f19f074e278c5acc7201700660,
title = "Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry",
abstract = "Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100{\%} sensitivity and 92.2{\%} specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100{\%} sensitivity and 100{\%} specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.",
author = "Viktor M{\aa}nsson and Gilsdorf, {Janet R.} and Gunnar Kahlmeter and Mogens Kilian and Kroll, {J. Simon} and Kristian Riesbeck and Fredrik Resman",
year = "2018",
month = "3",
day = "1",
doi = "10.3201/eid2403.170459",
language = "English",
volume = "24",
pages = "443--452",
journal = "Emerging Infectious Diseases (Print Edition)",
issn = "1080-6040",
publisher = "U.S. Department of Health and Human Services Centers for Disease Control and Prevention",
number = "3",

}

RIS

TY - JOUR

T1 - Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry

AU - Månsson, Viktor

AU - Gilsdorf, Janet R.

AU - Kahlmeter, Gunnar

AU - Kilian, Mogens

AU - Kroll, J. Simon

AU - Riesbeck, Kristian

AU - Resman, Fredrik

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.

AB - Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.

UR - http://www.scopus.com/inward/record.url?scp=85042311159&partnerID=8YFLogxK

U2 - 10.3201/eid2403.170459

DO - 10.3201/eid2403.170459

M3 - Journal article

VL - 24

SP - 443

EP - 452

JO - Emerging Infectious Diseases (Print Edition)

JF - Emerging Infectious Diseases (Print Edition)

SN - 1080-6040

IS - 3

ER -