Candidate gene association mapping for winter survival and spring regrowth in perennial ryegrass

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  • Xiaoqing Yu, Iowa State Univ, Iowa State University, Dept Agron
  • ,
  • Paula M. Pijut, USDAForest Serv, No Res Stn, Hardwood Tree Improvement & Regenerat Ctr
  • ,
  • Stephen Byrne
  • ,
  • Torben Asp
  • Guihua Bai, ARS, United States Department of Agriculture (USDA), USDA, Hard Winter Wheat Genet Res Unit
  • ,
  • Yiwei Jiang, Purdue Univ, Purdue University, Purdue University System, Dept Agron

Perennial iyegrass (Lolium perenne L) is a widely cultivated cool-season grass species because of its high quality for forage and turf. Susceptibility to freezing damage limits its further use in temperate zones. The objective of this study was to identify candidate genes significantly associated with winter survival and spring regrowth in a global collection of 192 perennial ryegrass accessions. Significant differences in winter survival (WS), percentage of canopy green cover (CGC), chlorophyll index (Chl), and normalized difference vegetation index (NDVI) were found among accessions. After controlling population structure, LpLEA3 encoding a late embryogenesis abundant group 3 protein and LpCAT encoding a catalase were associated with CGC and Chl, while LpMnSOD encoding a magnesium superoxide dismutase and LpChl Cu-ZnSOD encoding a chlorophyll copper zinc superoxide dismutase were associated with NDVI or Chl. Significant association was also discovered between C-repeat binding factor LpCBF1b and WS. Three sequence variations identified in LpCAT, LpMnSOD, and LpChl Cu-ZnSOD were synonymous substitutions, whereas one pair of adjacent single nucleotide polymorphisms (SNPs) in LpLEA3 and one SNP in LpCBF1b resulted in amino acid change. The results demonstrated that allelic variation in LpLEA3 and LpCBF1b was closely related to winter survival and spring regrowth in perennial ryegrass. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

OriginalsprogEngelsk
TidsskriftPlant Science
Vol/bind235
Sider (fra-til)37-45
Antal sider9
ISSN0168-9452
DOI
StatusUdgivet - jun. 2015

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