Can enzyme proximity accelerate cascade reactions?

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Dokumenter

DOI

  • Andrij Kuzmak, I. Franko National University of Lviv
  • ,
  • Sheiliza Carmali
  • Eric von Lieres, Forschungszentrum Jülich (FZJ)
  • ,
  • Alan J. Russell, Carnegie Mellon University
  • ,
  • Svyatoslav Kondrat, Forschungszentrum Jülich (FZJ), Institute of Physical Chemistry of the Polish Academy of Sciences

The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on the reaction velocity of the popular GOx-HRP cascade, particularly in the presence of a competing enzyme (catalase). Herein, we rationalize these experimental results quantitatively. We show that, in general, proximity channeling can enhance reaction velocity in the presence of competing enzymes, but in steady state a significant enhancement can only be achieved for diffusion-limited reactions or at high concentrations of competing enzymes. We provide simple equations to estimate the effect of channeling quantitatively and demonstrate that proximity can have a more pronounced effect under crowding conditions in vivo, particularly that crowding can enhance the overall rates of channeled cascade reactions.

OriginalsprogEngelsk
Artikelnummer455
TidsskriftScientific Reports
Vol/bind9
Nummer1
Antal sider7
ISSN2045-2322
DOI
StatusUdgivet - jan. 2019

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