Can a Charged Surfactant Unfold an Uncharged Protein?

Casper Højgaard; Henrik Vinther Sørensen; Jan Skov Pedersen; Jakob Rahr Winther; Daniel Erik Otzen

doi:10.1016/j.bpj.2018.10.022

Can a Charged Surfactant Unfold an Uncharged Protein?

Casper Højgaard, Henrik Vinther Sørensen, Jan Skov Pedersen, Jakob Rahr Winther, Daniel Erik Otzen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Does sodium dodecyl sulfate (SDS) denature proteins through electrostatic SDS-protein interactions? We show that a protein completely lacking charged side chains is unfolded by SDS in a manner similar to charged proteins, revealing that formal protein charges are not required for SDS-induced protein unfolding or binding.

Originalsprog	Engelsk
Tidsskrift	Biophysical Journal
Vol/bind	115
Nummer	11
Sider (fra-til)	2081-2086
Antal sider	6
ISSN	0006-3495
DOI	https://doi.org/10.1016/j.bpj.2018.10.022
Status	Udgivet - 4 dec. 2018

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10.1016/j.bpj.2018.10.022

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Citationsformater

@article{6fa1caedc5e24afa9e5882764ebfeb8b,

title = "Can a Charged Surfactant Unfold an Uncharged Protein?",

abstract = "Does sodium dodecyl sulfate (SDS) denature proteins through electrostatic SDS-protein interactions? We show that a protein completely lacking charged side chains is unfolded by SDS in a manner similar to charged proteins, revealing that formal protein charges are not required for SDS-induced protein unfolding or binding.",

author = "Casper H{\o}jgaard and S{\o}rensen, {Henrik Vinther} and Pedersen, {Jan Skov} and Winther, {Jakob Rahr} and Otzen, {Daniel Erik}",

year = "2018",

month = dec,

day = "4",

doi = "10.1016/j.bpj.2018.10.022",

language = "English",

volume = "115",

pages = "2081--2086",

journal = "Biophysical Journal",

issn = "0006-3495",