Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

Jesper Just; Yan Yan; Jean Farup; Peter Sieljacks; Mette Sloth; Morten Venø; Tingting Gu; Frank Vincenzo de Paoli; Jens Randel Nyengaard; Rikke Bæk; Malene Møller Jørgensen; Jørgen Kjems; Kristian Vissing; Kim Ryun Drasbek

doi:10.1038/s41598-020-62456-3

Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

Jesper Just, Yan Yan, Jean Farup, Peter Sieljacks, Mette Sloth, Morten Venø, Tingting Gu, Frank Vincenzo de Paoli, Jens Randel Nyengaard, Rikke Bæk, Malene Møller Jørgensen, Jørgen Kjems, Kristian Vissing, Kim Ryun Drasbek

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.

Originalsprog	Engelsk
Artikelnummer	5835
Tidsskrift	Scientific Reports
Vol/bind	10
Nummer	1
Antal sider	13
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-020-62456-3
Status	Udgivet - 2020

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10.1038/s41598-020-62456-3Licens: CC BY

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Just, J., Yan, Y., Farup, J., Sieljacks, P., Sloth, M., Venø, M., Gu, T., de Paoli, F. V., Nyengaard, J. R., Bæk, R., Jørgensen, M. M., Kjems, J., Vissing, K., & Drasbek, K. R. (2020). Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles. Scientific Reports, 10(1), Artikel 5835. https://doi.org/10.1038/s41598-020-62456-3

@article{1c4235ef4af0458aa28357ef1ce0f70b,

title = "Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles",

abstract = "Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.",

author = "Jesper Just and Yan Yan and Jean Farup and Peter Sieljacks and Mette Sloth and Morten Ven{\o} and Tingting Gu and {de Paoli}, {Frank Vincenzo} and Nyengaard, {Jens Randel} and Rikke B{\ae}k and J{\o}rgensen, {Malene M{\o}ller} and J{\o}rgen Kjems and Kristian Vissing and Drasbek, {Kim Ryun}",

year = "2020",

doi = "10.1038/s41598-020-62456-3",

language = "English",

volume = "10",

journal = "Scientific Reports",

issn = "2045-2322",

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Just, J, Yan, Y, Farup, J, Sieljacks, P, Sloth, M, Venø, M, Gu, T, de Paoli, FV , Nyengaard, JR, Bæk, R, Jørgensen, MM, Kjems, J , Vissing, K & Drasbek, KR 2020, 'Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles', Scientific Reports, bind 10, nr. 1, 5835. https://doi.org/10.1038/s41598-020-62456-3

Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles. / Just, Jesper; Yan, Yan; Farup, Jean et al.
I: Scientific Reports, Bind 10, Nr. 1, 5835, 2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

AU - Just, Jesper

AU - Yan, Yan

AU - Farup, Jean

AU - Sieljacks, Peter

AU - Sloth, Mette

AU - Venø, Morten

AU - Gu, Tingting

AU - de Paoli, Frank Vincenzo

AU - Nyengaard, Jens Randel

AU - Bæk, Rikke

AU - Jørgensen, Malene Møller

AU - Kjems, Jørgen

AU - Vissing, Kristian

AU - Drasbek, Kim Ryun

PY - 2020

Y1 - 2020

N2 - Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.

AB - Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.

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VL - 10

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 5835

ER -

Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

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