Biphasic Dose-Response and Hormetic Effects of Stress Hormone Hydrocortisone on Telomerase-Immortalised Human Bone Marrow Stem Cells in vitro

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Although high levels of stress hormones are associated with well-known negative health outcomes, their low levels can have health-promoting effects by virtue of the phenomenon of mild stress-induced hormesis. We have studied the effects of a wide range (between 100 nM and 150 μM) of hydrocortisone (HC) on human bone marrow stem cells in vitro. Telomerase-immortalized human mesenchymal stem cells (hTERT-MSC) were exposed to various doses of HC for different durations (1 to 6 days), and analysed for survival and mitochondrial metabolic activity by MTT assay, for cell migratory ability by a wound-healing assay, and for osteoblastic and adipogenic differentiation abilities in vitro. Our findings indicate that hTERT-MSC cells exposed to HC resulted in a biphasic hormetic dose-response in some measures but not all. Whereas the mitochondrial and metabolic MTT activity assay clearly showed low-level-stimulatory (between 01. and 1 µM) and high-level-inhibitory effects (from about 10 µM onwards), the cytostatic and differentiation-inducing effects were mostly linear at concentrations between 1 and 100 µM. Further long-term studies will elucidate whether chronic or intermittent exposure of human cells to stress hormones has physiologically beneficial hormetic effects.
OriginalsprogEngelsk
TidsskriftDose-Response
Vol/bind17
Nummer4
Sider (fra-til)1-9
Antal sider9
ISSN1559-3258
DOI
StatusUdgivet - dec. 2019

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