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Bioimaging techniques for subcellular localization of plant hemoglobins and measurement of hemoglobin-dependent nitric oxide scavenging in planta

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Bioimaging techniques for subcellular localization of plant hemoglobins and measurement of hemoglobin-dependent nitric oxide scavenging in planta. / Hebelstrup, Kim H; Østergaard-Jensen, Erik; Hill, Robert D.

I: Methods in Enzymology, Bind 437, 2008, s. 595-604.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{c8f229b0e24411dd8fc3000ea68e967b,
title = "Bioimaging techniques for subcellular localization of plant hemoglobins and measurement of hemoglobin-dependent nitric oxide scavenging in planta",
abstract = "Plant hemoglobins are ubiquitous in all plant families. They are expressed at low levels in specific tissues. Several studies have established that plant hemoglobins are scavengers of nitric oxide (NO) and that varying the endogenous level of hemoglobin in plant cells negatively modulates bioactivity of NO generated under hypoxic conditions or during cellular signaling. Earlier methods for determination of hemoglobin-dependent scavenging in planta were based on measuring activity in whole plants or organs. Plant hemoglobins do not contain specific organelle localization signals; however, earlier reports on plant hemoglobin have demonstrated either cytosolic or nuclear localization, depending on the method or cell type investigated. We have developed two bioimaging techniques: one for visualization of hemoglobin-catalyzed scavenging of NO in specific cells and another for visualization of subcellular localization of green fluorescent protein-tagged plant hemoglobins in transformed Arabidopsis thaliana plants.",
keywords = "Arabidopsis, Arabidopsis Proteins, Free Radical Scavengers, Green Fluorescent Proteins, Hemoglobins, Microscopy, Confocal, Microscopy, Fluorescence, Nitric Oxide, Plant Proteins, Plants, Protein Engineering, Recombinant Fusion Proteins, Tissue Distribution",
author = "Hebelstrup, {Kim H} and Erik {\O}stergaard-Jensen and Hill, {Robert D}",
year = "2008",
doi = "10.1016/S0076-6879(07)37030-4",
language = "English",
volume = "437",
pages = "595--604",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Bioimaging techniques for subcellular localization of plant hemoglobins and measurement of hemoglobin-dependent nitric oxide scavenging in planta

AU - Hebelstrup, Kim H

AU - Østergaard-Jensen, Erik

AU - Hill, Robert D

PY - 2008

Y1 - 2008

N2 - Plant hemoglobins are ubiquitous in all plant families. They are expressed at low levels in specific tissues. Several studies have established that plant hemoglobins are scavengers of nitric oxide (NO) and that varying the endogenous level of hemoglobin in plant cells negatively modulates bioactivity of NO generated under hypoxic conditions or during cellular signaling. Earlier methods for determination of hemoglobin-dependent scavenging in planta were based on measuring activity in whole plants or organs. Plant hemoglobins do not contain specific organelle localization signals; however, earlier reports on plant hemoglobin have demonstrated either cytosolic or nuclear localization, depending on the method or cell type investigated. We have developed two bioimaging techniques: one for visualization of hemoglobin-catalyzed scavenging of NO in specific cells and another for visualization of subcellular localization of green fluorescent protein-tagged plant hemoglobins in transformed Arabidopsis thaliana plants.

AB - Plant hemoglobins are ubiquitous in all plant families. They are expressed at low levels in specific tissues. Several studies have established that plant hemoglobins are scavengers of nitric oxide (NO) and that varying the endogenous level of hemoglobin in plant cells negatively modulates bioactivity of NO generated under hypoxic conditions or during cellular signaling. Earlier methods for determination of hemoglobin-dependent scavenging in planta were based on measuring activity in whole plants or organs. Plant hemoglobins do not contain specific organelle localization signals; however, earlier reports on plant hemoglobin have demonstrated either cytosolic or nuclear localization, depending on the method or cell type investigated. We have developed two bioimaging techniques: one for visualization of hemoglobin-catalyzed scavenging of NO in specific cells and another for visualization of subcellular localization of green fluorescent protein-tagged plant hemoglobins in transformed Arabidopsis thaliana plants.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Free Radical Scavengers

KW - Green Fluorescent Proteins

KW - Hemoglobins

KW - Microscopy, Confocal

KW - Microscopy, Fluorescence

KW - Nitric Oxide

KW - Plant Proteins

KW - Plants

KW - Protein Engineering

KW - Recombinant Fusion Proteins

KW - Tissue Distribution

U2 - 10.1016/S0076-6879(07)37030-4

DO - 10.1016/S0076-6879(07)37030-4

M3 - Journal article

C2 - 18433649

VL - 437

SP - 595

EP - 604

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

ER -