Biochemical and rapid molecular analyses to identify glyphosate resistance in Lolium spp.

Maria Gerakari, Nikolina Cheimona, Eleni Tani*, Ilias Travlos, Demosthenis Chachalis, Donato Loddo, Solvejg Kopp Mathiassen, Thomas K. Gitsopoulos, Laura Scarabel, Silvia Panozzo, Michael Kristensen, Per Kudsk, Maurizio Sattin

*Corresponding author af dette arbejde

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Abstract

Lolium spp. are troublesome weeds mainly found in winter cereal crops worldwide, includ-ing Europe. In recent years resistant mechanisms have been evolved to several important herbicides. In this study we investigated the mechanisms responsible for conferring glyphosate resistance in some Lolium spp. populations. A holistic approach was used, based on dose-response experiments, determination of shikimic acid concentration in plant leaf tissue, as well as molecular analyses. More specifically, in three Lolium spp. populations the existence of a mutation in the Pro-106 codon of the 5-enolpyruvylshikimate-3 phosphate synthase (EPSPS) gene was investigated as well as the relative transcript levels of four ABC-transporter genes were monitored at three time points after glyphosate application. The results demonstrated that glyphosate resistance is a multifactor phenomenon. Relative transcript levels of the ABC-transporter genes were abundant at very early time points after glyphosate treatments. Dose-response experiments and shikimate analyses were in accordance with the findings of the quantitative PCR (qPCR) analyses. We suggest that relative expression ratio of ABC-transporter genes can be a useful tool to rapidly identify Lolium spp. populations resistant to glyphosate.

OriginalsprogEngelsk
Artikelnummer40
TidsskriftAgronomy
Vol/bind12
Nummer1
Antal sider12
ISSN2073-4395
DOI
StatusUdgivet - jan. 2022

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