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Biochemical and morphological responses to post-hepatectomy liver failure in rats

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

The upper limit for partial hepatectomy (PH) in rats is 90%, which is associated with an increased risk of post-hepatectomy liver failure (PHLF), correlating with high mortality. Sixty-eight rats were randomized to 90% PH, sham operation, or no surgery. Further block randomization was performed to determine the time of euthanasia, whether 12, 24, or 48 h after surgery. A general distress score (GDS) was calculated to distinguish between rats with reversible (GDS < 10) and irreversible PHLF (GDS ≥ 10). At euthanasia, the liver remnant and blood were collected. Liver-specific biochemistry and regeneration ratio were measured. Hepatocyte proliferation and volume were estimated using stereological methods. All rats subjected to 90% experienced biochemical PHLF. The biochemical and morphological liver responses did not differ between the groups until 48 h after surgery. At 48 h, liver regeneration and function were significantly improved in survivors. The peak mean regeneration ratio was 15% for rats with irreversible PHLF compared to 26% for rats with reversible PHLF. The 90% PH rat model was associated with PHLF and high mortality. Irreversible PHLF was characterized by impaired liver regeneration capacity and an insufficient ability to metabolize ammonia.

OriginalsprogEngelsk
Artikelnummer13544
TidsskriftScientific Reports
Vol/bind13
Nummer1
ISSN2045-2322
DOI
StatusUdgivet - aug. 2023

Bibliografisk note

Funding Information:
The authors thank Arvid Nilssons Foundation, Købmand Sven Hansen og Hustru Ina Hansens Foundation, Fabrikant Einar Willumsens Foundation, Familien Hede Nielsens Foundation, P.A. Messerschmidt og Hustrus Foundation, and AP Møller Foundation for funding the research. Further, we thank Jeanette Bæhr Georgsen and Trine Werenberg Mikkelsen for excellent technical assistance.

Publisher Copyright:
© 2023, Springer Nature Limited.

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