Bioactive coronary stent coating based on layer-by-layer technology for siRNA release

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Bioactive coronary stent coating based on layer-by-layer technology for siRNA release. / Hossfeld, S.; Nolte, A.; Hartmann, H.; Recke, M.; Schaller, M.; Walker, T.; Kjems, Jørgen; Schlosshauer, B.; Stoll, D.; Wendel, H.-P.; Krastev, R.

I: Acta Biomaterialia, Bind 9, Nr. 5, 05.2013, s. 6741-6752.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hossfeld, S, Nolte, A, Hartmann, H, Recke, M, Schaller, M, Walker, T, Kjems, J, Schlosshauer, B, Stoll, D, Wendel, H-P & Krastev, R 2013, 'Bioactive coronary stent coating based on layer-by-layer technology for siRNA release', Acta Biomaterialia, bind 9, nr. 5, s. 6741-6752. https://doi.org/10.1016/j.actbio.2013.01.013

APA

Hossfeld, S., Nolte, A., Hartmann, H., Recke, M., Schaller, M., Walker, T., ... Krastev, R. (2013). Bioactive coronary stent coating based on layer-by-layer technology for siRNA release. Acta Biomaterialia, 9(5), 6741-6752. https://doi.org/10.1016/j.actbio.2013.01.013

CBE

Hossfeld S, Nolte A, Hartmann H, Recke M, Schaller M, Walker T, Kjems J, Schlosshauer B, Stoll D, Wendel H-P, Krastev R. 2013. Bioactive coronary stent coating based on layer-by-layer technology for siRNA release. Acta Biomaterialia. 9(5):6741-6752. https://doi.org/10.1016/j.actbio.2013.01.013

MLA

Vancouver

Hossfeld S, Nolte A, Hartmann H, Recke M, Schaller M, Walker T o.a. Bioactive coronary stent coating based on layer-by-layer technology for siRNA release. Acta Biomaterialia. 2013 maj;9(5):6741-6752. https://doi.org/10.1016/j.actbio.2013.01.013

Author

Hossfeld, S. ; Nolte, A. ; Hartmann, H. ; Recke, M. ; Schaller, M. ; Walker, T. ; Kjems, Jørgen ; Schlosshauer, B. ; Stoll, D. ; Wendel, H.-P. ; Krastev, R. / Bioactive coronary stent coating based on layer-by-layer technology for siRNA release. I: Acta Biomaterialia. 2013 ; Bind 9, Nr. 5. s. 6741-6752.

Bibtex

@article{2e67320964154d18acb5d27220179eff,
title = "Bioactive coronary stent coating based on layer-by-layer technology for siRNA release",
abstract = "One procedure to treat stenotic coronary arteries is the percutaneous transluminal coronary angioplasty (PTCA). In recent years, drug-eluting stents (DESs) have demonstrated elaborate ways to improve outcomes of intravascular interventions. To enhance DESs, the idea has evolved to design stents that elute specific small interfering RNA (siRNA) for better vascular wall regeneration. Layer-by-layer (LbL) technology offers the possibility of incorporating siRNA nanoplexes (NPs) to achieve bioactive medical implant coatings. The LbL technique was used to achieve hyaluronic acid/chitosan (HA/Chi) films with incorporated Chi-siRNA NPs. The multilayer growth was monitored by quartz crystal microbalance. The coating on the stents and its thickness were analyzed using fluorescence and scanning electron microscopy. All stents showed a homogeneous coating, and the polyelectrolyte multilayers (PEMs) were not disrupted after ethylene oxide sterilization or expansion. The in vitro uptake of fluorescent-labeled NPs from PEMs in primary human endothelial cells (ECs) was analyzed by flow cytometry for 2, 6 and 9 days. Furthermore, stents coated with HA/Chi and Chi-siRNA NPs were expanded into porcine arteries and showed ex vivo delivery of NPs. The films showed no critical results in terms of hemocompatibility. This study demonstrates that Chi-siRNA NPs can be incorporated into PEMs consisting of HA and Chi. We conclude that the NPs were delivered to ECs under in vitro conditions. Furthermore, under ex vivo conditions, NPs were transferred into porcine artery walls. Due to their good hemocompatibility, they might make an innovative tool for achieving bioactive coatings for coronary stents",
keywords = "Endothelial cells",
author = "S. Hossfeld and A. Nolte and H. Hartmann and M. Recke and M. Schaller and T. Walker and J{\o}rgen Kjems and B. Schlosshauer and D. Stoll and H.-P. Wendel and R. Krastev",
year = "2013",
month = "5",
doi = "10.1016/j.actbio.2013.01.013",
language = "English",
volume = "9",
pages = "6741--6752",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "5",

}

RIS

TY - JOUR

T1 - Bioactive coronary stent coating based on layer-by-layer technology for siRNA release

AU - Hossfeld, S.

AU - Nolte, A.

AU - Hartmann, H.

AU - Recke, M.

AU - Schaller, M.

AU - Walker, T.

AU - Kjems, Jørgen

AU - Schlosshauer, B.

AU - Stoll, D.

AU - Wendel, H.-P.

AU - Krastev, R.

PY - 2013/5

Y1 - 2013/5

N2 - One procedure to treat stenotic coronary arteries is the percutaneous transluminal coronary angioplasty (PTCA). In recent years, drug-eluting stents (DESs) have demonstrated elaborate ways to improve outcomes of intravascular interventions. To enhance DESs, the idea has evolved to design stents that elute specific small interfering RNA (siRNA) for better vascular wall regeneration. Layer-by-layer (LbL) technology offers the possibility of incorporating siRNA nanoplexes (NPs) to achieve bioactive medical implant coatings. The LbL technique was used to achieve hyaluronic acid/chitosan (HA/Chi) films with incorporated Chi-siRNA NPs. The multilayer growth was monitored by quartz crystal microbalance. The coating on the stents and its thickness were analyzed using fluorescence and scanning electron microscopy. All stents showed a homogeneous coating, and the polyelectrolyte multilayers (PEMs) were not disrupted after ethylene oxide sterilization or expansion. The in vitro uptake of fluorescent-labeled NPs from PEMs in primary human endothelial cells (ECs) was analyzed by flow cytometry for 2, 6 and 9 days. Furthermore, stents coated with HA/Chi and Chi-siRNA NPs were expanded into porcine arteries and showed ex vivo delivery of NPs. The films showed no critical results in terms of hemocompatibility. This study demonstrates that Chi-siRNA NPs can be incorporated into PEMs consisting of HA and Chi. We conclude that the NPs were delivered to ECs under in vitro conditions. Furthermore, under ex vivo conditions, NPs were transferred into porcine artery walls. Due to their good hemocompatibility, they might make an innovative tool for achieving bioactive coatings for coronary stents

AB - One procedure to treat stenotic coronary arteries is the percutaneous transluminal coronary angioplasty (PTCA). In recent years, drug-eluting stents (DESs) have demonstrated elaborate ways to improve outcomes of intravascular interventions. To enhance DESs, the idea has evolved to design stents that elute specific small interfering RNA (siRNA) for better vascular wall regeneration. Layer-by-layer (LbL) technology offers the possibility of incorporating siRNA nanoplexes (NPs) to achieve bioactive medical implant coatings. The LbL technique was used to achieve hyaluronic acid/chitosan (HA/Chi) films with incorporated Chi-siRNA NPs. The multilayer growth was monitored by quartz crystal microbalance. The coating on the stents and its thickness were analyzed using fluorescence and scanning electron microscopy. All stents showed a homogeneous coating, and the polyelectrolyte multilayers (PEMs) were not disrupted after ethylene oxide sterilization or expansion. The in vitro uptake of fluorescent-labeled NPs from PEMs in primary human endothelial cells (ECs) was analyzed by flow cytometry for 2, 6 and 9 days. Furthermore, stents coated with HA/Chi and Chi-siRNA NPs were expanded into porcine arteries and showed ex vivo delivery of NPs. The films showed no critical results in terms of hemocompatibility. This study demonstrates that Chi-siRNA NPs can be incorporated into PEMs consisting of HA and Chi. We conclude that the NPs were delivered to ECs under in vitro conditions. Furthermore, under ex vivo conditions, NPs were transferred into porcine artery walls. Due to their good hemocompatibility, they might make an innovative tool for achieving bioactive coatings for coronary stents

KW - Endothelial cells

U2 - 10.1016/j.actbio.2013.01.013

DO - 10.1016/j.actbio.2013.01.013

M3 - Journal article

C2 - 23333865

VL - 9

SP - 6741

EP - 6752

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 5

ER -