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Best practice standards for circular RNA research

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DOI

  • Anne Færch Nielsen
  • Albrecht Bindereif, Justus Liebig University Giessen
  • ,
  • Irene Bozzoni, University of Rome La Sapienza
  • ,
  • Mor Hanan, Hebrew University of Jerusalem
  • ,
  • Thomas Birkballe Hansen
  • ,
  • Manuel Irimia, Barcelona Institute of Science and Technology, Pompeu Fabra University, ICREA
  • ,
  • Sebastian Kadener, Brandeis University
  • ,
  • Lasse Sommer Kristensen
  • Ivano Legnini, Max Delbrück Center for Molecular Medicine in the Helmholtz Association
  • ,
  • Mariangela Morlando, University of Perugia
  • ,
  • Morten Tobias Jarlstad Olesen
  • R Jeroen Pasterkamp, Utrecht University
  • ,
  • Stephan Preibisch, Max Delbrück Center for Molecular Medicine in the Helmholtz Association
  • ,
  • Nikolaus Rajewsky, Max Delbrück Center for Molecular Medicine in the Helmholtz Association
  • ,
  • Christin Sünkel, Max Delbrück Center for Molecular Medicine in the Helmholtz Association
  • ,
  • Jørgen Kjems

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

OriginalsprogEngelsk
TidsskriftNature Methods
Vol/bind19
Nummer10
Sider (fra-til)1208-1220
Antal sider13
ISSN1548-7091
DOI
StatusUdgivet - okt. 2022

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