Best practice standards for circular RNA research

Anne Færch Nielsen, Albrecht Bindereif, Irene Bozzoni, Mor Hanan, Thomas Birkballe Hansen, Manuel Irimia, Sebastian Kadener, Lasse Sommer Kristensen, Ivano Legnini, Mariangela Morlando, Morten Tobias Jarlstad Olesen, R Jeroen Pasterkamp, Stephan Preibisch, Nikolaus Rajewsky, Christin Sünkel, Jørgen Kjems*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

OriginalsprogEngelsk
TidsskriftNature Methods
Vol/bind19
Nummer10
Sider (fra-til)1208-1220
Antal sider13
ISSN1548-7091
DOI
StatusUdgivet - okt. 2022

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