Barley starch bioengineering for high phosphate and amylose

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisKonferenceabstrakt i tidsskriftForskningpeer review

Standard

Barley starch bioengineering for high phosphate and amylose. / Blennow, Per Gunnar Andreas; Carciofi, Massimiliano; Shaik, Shahnoor Sultana; Jensen, Susanne Langgård; Svensson, Jan Tommy; Holm, Preben Bach; Hebelstrup, Kim.

I: Cereal Foods World, Bind 56, Nr. 4, 2011, s. A15.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisKonferenceabstrakt i tidsskriftForskningpeer review

Harvard

Blennow, PGA, Carciofi, M, Shaik, SS, Jensen, SL, Svensson, JT, Holm, PB & Hebelstrup, K 2011, 'Barley starch bioengineering for high phosphate and amylose' Cereal Foods World, bind 56, nr. 4, s. A15.

APA

Blennow, P. G. A., Carciofi, M., Shaik, S. S., Jensen, S. L., Svensson, J. T., Holm, P. B., & Hebelstrup, K. (2011). Barley starch bioengineering for high phosphate and amylose. Cereal Foods World, 56(4), A15.

CBE

Blennow PGA, Carciofi M, Shaik SS, Jensen SL, Svensson JT, Holm PB, Hebelstrup K. 2011. Barley starch bioengineering for high phosphate and amylose. Cereal Foods World. 56(4):A15.

MLA

Blennow, Per Gunnar Andreas o.a.. "Barley starch bioengineering for high phosphate and amylose". Cereal Foods World. 2011, 56(4). A15.

Vancouver

Blennow PGA, Carciofi M, Shaik SS, Jensen SL, Svensson JT, Holm PB o.a. Barley starch bioengineering for high phosphate and amylose. Cereal Foods World. 2011;56(4):A15.

Author

Blennow, Per Gunnar Andreas ; Carciofi, Massimiliano ; Shaik, Shahnoor Sultana ; Jensen, Susanne Langgård ; Svensson, Jan Tommy ; Holm, Preben Bach ; Hebelstrup, Kim. / Barley starch bioengineering for high phosphate and amylose. I: Cereal Foods World. 2011 ; Bind 56, Nr. 4. s. A15.

Bibtex

@article{bf9001f83af847b6bfd979f5a80781c1,
title = "Barley starch bioengineering for high phosphate and amylose",
abstract = "Starch is a biological polymer that can be industrially produced in massive amounts in a very pure form. Cereals is the main source for starch production and any improvement of the starch fraction can have a tremendous impact in food and feed applications. Barley ranks number four among cereal crops and barley is a genetically very well characterized. Aiming at producing new starch qualities in the cereal system, we used RNAi and overexpression strategies to produce pure amylose and high-phosphate starch, respectively, using the barley kernel as a polymer factory. By simultaneous silencing of the three genes encoding the starch-branching enzymes SBEI, SBEIIa, and SBEIIb using a triple RNAi chimeric hairpin construct we generated a virtually amylopectin-free barley. The grains of the transgenic lines were shrunken and had a yield of around 80{\%} of the control line. The starch granules were irregular and showed no distinct melting enthalpy and very weak X-ray scattering. Hyperphosphorylated barley starch was achieved by endosperm specific overexpression of the potato glucan water dikinase1 (StGWD1). The content of phosphate esters in this starch was tenfold higher than the control lines. Amylose content was not affected but the starch granules had several pores on the surfaces and a decreased melting enthalpy indicating starch degradation stimulated by phosphate-induced amorphisation. Our work demonstrates the feasibility of starch bioengineering to produce completely novels starch-based polymers implementing two new strategies for in planta starch bioengineering of cereals.",
author = "Blennow, {Per Gunnar Andreas} and Massimiliano Carciofi and Shaik, {Shahnoor Sultana} and Jensen, {Susanne Langg{\aa}rd} and Svensson, {Jan Tommy} and Holm, {Preben Bach} and Kim Hebelstrup",
note = "Supplement to Cereal Foods World vol. 56 No 4",
year = "2011",
language = "English",
volume = "56",
pages = "A15",
journal = "Cereal Foods World",
issn = "0146-6283",
publisher = "American Association of Cereal Chemists, Inc.",
number = "4",

}

RIS

TY - ABST

T1 - Barley starch bioengineering for high phosphate and amylose

AU - Blennow, Per Gunnar Andreas

AU - Carciofi, Massimiliano

AU - Shaik, Shahnoor Sultana

AU - Jensen, Susanne Langgård

AU - Svensson, Jan Tommy

AU - Holm, Preben Bach

AU - Hebelstrup, Kim

N1 - Supplement to Cereal Foods World vol. 56 No 4

PY - 2011

Y1 - 2011

N2 - Starch is a biological polymer that can be industrially produced in massive amounts in a very pure form. Cereals is the main source for starch production and any improvement of the starch fraction can have a tremendous impact in food and feed applications. Barley ranks number four among cereal crops and barley is a genetically very well characterized. Aiming at producing new starch qualities in the cereal system, we used RNAi and overexpression strategies to produce pure amylose and high-phosphate starch, respectively, using the barley kernel as a polymer factory. By simultaneous silencing of the three genes encoding the starch-branching enzymes SBEI, SBEIIa, and SBEIIb using a triple RNAi chimeric hairpin construct we generated a virtually amylopectin-free barley. The grains of the transgenic lines were shrunken and had a yield of around 80% of the control line. The starch granules were irregular and showed no distinct melting enthalpy and very weak X-ray scattering. Hyperphosphorylated barley starch was achieved by endosperm specific overexpression of the potato glucan water dikinase1 (StGWD1). The content of phosphate esters in this starch was tenfold higher than the control lines. Amylose content was not affected but the starch granules had several pores on the surfaces and a decreased melting enthalpy indicating starch degradation stimulated by phosphate-induced amorphisation. Our work demonstrates the feasibility of starch bioengineering to produce completely novels starch-based polymers implementing two new strategies for in planta starch bioengineering of cereals.

AB - Starch is a biological polymer that can be industrially produced in massive amounts in a very pure form. Cereals is the main source for starch production and any improvement of the starch fraction can have a tremendous impact in food and feed applications. Barley ranks number four among cereal crops and barley is a genetically very well characterized. Aiming at producing new starch qualities in the cereal system, we used RNAi and overexpression strategies to produce pure amylose and high-phosphate starch, respectively, using the barley kernel as a polymer factory. By simultaneous silencing of the three genes encoding the starch-branching enzymes SBEI, SBEIIa, and SBEIIb using a triple RNAi chimeric hairpin construct we generated a virtually amylopectin-free barley. The grains of the transgenic lines were shrunken and had a yield of around 80% of the control line. The starch granules were irregular and showed no distinct melting enthalpy and very weak X-ray scattering. Hyperphosphorylated barley starch was achieved by endosperm specific overexpression of the potato glucan water dikinase1 (StGWD1). The content of phosphate esters in this starch was tenfold higher than the control lines. Amylose content was not affected but the starch granules had several pores on the surfaces and a decreased melting enthalpy indicating starch degradation stimulated by phosphate-induced amorphisation. Our work demonstrates the feasibility of starch bioengineering to produce completely novels starch-based polymers implementing two new strategies for in planta starch bioengineering of cereals.

M3 - Conference abstract in journal

VL - 56

SP - A15

JO - Cereal Foods World

JF - Cereal Foods World

SN - 0146-6283

IS - 4

ER -