Bacterial efflux pumps excrete SYTO™ dyes and lead to false-negative staining results

Gabriel Antonio Salvador Minero; Peter Bundgaard Larsen; Marie Eline Hoppe; Rikke Louise Meyer

doi:10.1039/d3an02112b

Bacterial efflux pumps excrete SYTO™ dyes and lead to false-negative staining results

Gabriel Antonio Salvador Minero, Peter Bundgaard Larsen, Marie Eline Hoppe, Rikke Louise Meyer^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

3 Citationer (Scopus)

Abstract

Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO™ dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO™ dyes should be used with caution on live samples.

Originalsprog	Engelsk
Tidsskrift	Analyst
Vol/bind	149
Nummer	8
Sider (fra-til)	2232-2235
Antal sider	4
ISSN	0003-2654
DOI	https://doi.org/10.1039/d3an02112b
Status	Udgivet - feb. 2024

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title = "Bacterial efflux pumps excrete SYTO{\texttrademark} dyes and lead to false-negative staining results",

abstract = "Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO{\texttrademark} dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO{\texttrademark} dyes should be used with caution on live samples.",

author = "Minero, {Gabriel Antonio Salvador} and Larsen, {Peter Bundgaard} and Hoppe, {Marie Eline} and Meyer, {Rikke Louise}",

note = "Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",

year = "2024",

month = feb,

doi = "10.1039/d3an02112b",

language = "English",

volume = "149",

pages = "2232--2235",

journal = "Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

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T1 - Bacterial efflux pumps excrete SYTO™ dyes and lead to false-negative staining results

AU - Minero, Gabriel Antonio Salvador

AU - Larsen, Peter Bundgaard

AU - Hoppe, Marie Eline

AU - Meyer, Rikke Louise

PY - 2024/2

Y1 - 2024/2

N2 - Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO™ dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO™ dyes should be used with caution on live samples.

AB - Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO™ dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO™ dyes should be used with caution on live samples.

UR - http://www.scopus.com/inward/record.url?scp=85187382125&partnerID=8YFLogxK

U2 - 10.1039/d3an02112b

DO - 10.1039/d3an02112b

M3 - Journal article

C2 - 38445898

AN - SCOPUS:85187382125

SN - 0003-2654

VL - 149

SP - 2232

EP - 2235

JO - Analyst

JF - Analyst

IS - 8

ER -

Bacterial efflux pumps excrete SYTO™ dyes and lead to false-negative staining results

Abstract

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