Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry

Marco Sette, Laura Anne Johnson, Ralph Jimenez, Frans A A Mulder*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Abstract

mCherry is one of the most successfully applied monomeric red fluorescent proteins (RFPs) for in vivo and in vitro imaging. However, questions pertaining to the photostability of the RFPs remain and rational further engineering of their photostability requires information about the fluorescence quenching mechanism in solution. To this end, NMR spectroscopic investigations might be helpful, and we present the near-complete backbone NMR chemical shift assignment to aid in this pursuit.

OriginalsprogEngelsk
TidsskriftBiomolecular NMR Assignments
Vol/bind17
Nummer2
Sider (fra-til)243-247
Antal sider5
ISSN1874-2718
DOI
StatusUdgivet - dec. 2023

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