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B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity

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B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity. / Brudek, Tomasz; Christensen, Tove; Aagaard, Lars; Petersen, Thor; Hansen, Hans J; Møller-Larsen, Anné.

I: Retrovirology, Bind 6, 2009, s. 104.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{cd0ca7d00f3b11dfb95d000ea68e967b,
title = "B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity",
abstract = "BACKGROUND: The etiology of the neurogenerative disease multiple sclerosis (MS) is unknown. The leading hypotheses suggest that MS is the result of exposure of genetically susceptible individuals to certain environmental factor(s). Herpesviruses and human endogenous retroviruses (HERVs) represent potentially important factors in MS development. Herpesviruses can activate HERVs, and HERVs are activated in MS patients. RESULTS: Using flow cytometry, we have analyzed HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease, and from control individuals. We have also analyzed serum antibody levels to the expressed HERV-H and HERV-W Env epitopes. We found a significantly higher expression of HERV-H and HERV-W Env epitopes on B cells and monocytes from patients with active MS compared with patients with stable MS or control individuals. Furthermore, patients with active disease had relatively higher numbers of B cells in the PBMC population, and higher antibody reactivities towards HERV-H Env and HERV-W Env epitopes. The higher antibody reactivities in sera from patients with active MS correlate with the higher levels of HERV-H Env and HERV-W Env expression on B cells and monocytes. We did not find such correlations for stable MS patients or for controls. CONCLUSION: These findings indicate that both HERV-H Env and HERV-W Env are expressed in higher quantities on the surface of B cells and monocytes in patients with active MS, and that the expression of these proteins may be associated with exacerbation of the disease.",
keywords = "Adult, Aged, Antibodies, Viral, B-Lymphocytes, Cells, Cultured, Endogenous Retroviruses, Epitopes, Female, Gene Products, env, Humans, Male, Middle Aged, Monocytes, Multiple Sclerosis",
author = "Tomasz Brudek and Tove Christensen and Lars Aagaard and Thor Petersen and Hansen, {Hans J} and Ann{\'e} M{\o}ller-Larsen",
year = "2009",
doi = "10.1186/1742-4690-6-104",
language = "English",
volume = "6",
pages = "104",
journal = "Retrovirology",
issn = "1742-4690",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity

AU - Brudek, Tomasz

AU - Christensen, Tove

AU - Aagaard, Lars

AU - Petersen, Thor

AU - Hansen, Hans J

AU - Møller-Larsen, Anné

PY - 2009

Y1 - 2009

N2 - BACKGROUND: The etiology of the neurogenerative disease multiple sclerosis (MS) is unknown. The leading hypotheses suggest that MS is the result of exposure of genetically susceptible individuals to certain environmental factor(s). Herpesviruses and human endogenous retroviruses (HERVs) represent potentially important factors in MS development. Herpesviruses can activate HERVs, and HERVs are activated in MS patients. RESULTS: Using flow cytometry, we have analyzed HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease, and from control individuals. We have also analyzed serum antibody levels to the expressed HERV-H and HERV-W Env epitopes. We found a significantly higher expression of HERV-H and HERV-W Env epitopes on B cells and monocytes from patients with active MS compared with patients with stable MS or control individuals. Furthermore, patients with active disease had relatively higher numbers of B cells in the PBMC population, and higher antibody reactivities towards HERV-H Env and HERV-W Env epitopes. The higher antibody reactivities in sera from patients with active MS correlate with the higher levels of HERV-H Env and HERV-W Env expression on B cells and monocytes. We did not find such correlations for stable MS patients or for controls. CONCLUSION: These findings indicate that both HERV-H Env and HERV-W Env are expressed in higher quantities on the surface of B cells and monocytes in patients with active MS, and that the expression of these proteins may be associated with exacerbation of the disease.

AB - BACKGROUND: The etiology of the neurogenerative disease multiple sclerosis (MS) is unknown. The leading hypotheses suggest that MS is the result of exposure of genetically susceptible individuals to certain environmental factor(s). Herpesviruses and human endogenous retroviruses (HERVs) represent potentially important factors in MS development. Herpesviruses can activate HERVs, and HERVs are activated in MS patients. RESULTS: Using flow cytometry, we have analyzed HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease, and from control individuals. We have also analyzed serum antibody levels to the expressed HERV-H and HERV-W Env epitopes. We found a significantly higher expression of HERV-H and HERV-W Env epitopes on B cells and monocytes from patients with active MS compared with patients with stable MS or control individuals. Furthermore, patients with active disease had relatively higher numbers of B cells in the PBMC population, and higher antibody reactivities towards HERV-H Env and HERV-W Env epitopes. The higher antibody reactivities in sera from patients with active MS correlate with the higher levels of HERV-H Env and HERV-W Env expression on B cells and monocytes. We did not find such correlations for stable MS patients or for controls. CONCLUSION: These findings indicate that both HERV-H Env and HERV-W Env are expressed in higher quantities on the surface of B cells and monocytes in patients with active MS, and that the expression of these proteins may be associated with exacerbation of the disease.

KW - Adult

KW - Aged

KW - Antibodies, Viral

KW - B-Lymphocytes

KW - Cells, Cultured

KW - Endogenous Retroviruses

KW - Epitopes

KW - Female

KW - Gene Products, env

KW - Humans

KW - Male

KW - Middle Aged

KW - Monocytes

KW - Multiple Sclerosis

U2 - 10.1186/1742-4690-6-104

DO - 10.1186/1742-4690-6-104

M3 - Journal article

C2 - 19917105

VL - 6

SP - 104

JO - Retrovirology

JF - Retrovirology

SN - 1742-4690

ER -