Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers

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Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers. / Afzelius, Pia; Alstrup, Aage Kristian Olsen; Nielsen, Ole Lerberg; Nielsen, Karin Michaelsen; Jensen, Svend Borup.

I: Molecules, Bind 25, Nr. 18, 4329, 09.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Afzelius, Pia ; Alstrup, Aage Kristian Olsen ; Nielsen, Ole Lerberg ; Nielsen, Karin Michaelsen ; Jensen, Svend Borup. / Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers. I: Molecules. 2020 ; Bind 25, Nr. 18.

Bibtex

@article{56c40e48c5ec4bcda21c1f370b24b602,
title = "Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers",
abstract = "Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.",
keywords = "animal experimentation, osteomyelitis, PET/CT, pigs, [18F]FDG, [18F]NaF, [68Ga]Ga-DOTA-GSGK-A11 [68Ga]Ga-DOTA-Siglec-9, [68Ga]Ga-DOTA-K-A9, [68Ga]Ga-ubiquicidin",
author = "Pia Afzelius and Alstrup, {Aage Kristian Olsen} and Nielsen, {Ole Lerberg} and Nielsen, {Karin Michaelsen} and Jensen, {Svend Borup}",
year = "2020",
month = sep,
doi = "10.3390/molecules25184329",
language = "English",
volume = "25",
journal = "Molecules",
issn = "1420-3049",
publisher = "M D P I AG",
number = "18",

}

RIS

TY - JOUR

T1 - Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers

AU - Afzelius, Pia

AU - Alstrup, Aage Kristian Olsen

AU - Nielsen, Ole Lerberg

AU - Nielsen, Karin Michaelsen

AU - Jensen, Svend Borup

PY - 2020/9

Y1 - 2020/9

N2 - Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.

AB - Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.

KW - animal experimentation

KW - osteomyelitis

KW - PET/CT

KW - pigs

KW - [18F]FDG

KW - [18F]NaF

KW - [68Ga]Ga-DOTA-GSGK-A11 [68Ga]Ga-DOTA-Siglec-9

KW - [68Ga]Ga-DOTA-K-A9

KW - [68Ga]Ga-ubiquicidin

UR - http://www.scopus.com/inward/record.url?scp=85091540099&partnerID=8YFLogxK

U2 - 10.3390/molecules25184329

DO - 10.3390/molecules25184329

M3 - Journal article

C2 - 32967275

AN - SCOPUS:85091540099

VL - 25

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 18

M1 - 4329

ER -