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Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers. / Afzelius, Pia; Alstrup, Aage Kristian Olsen; Nielsen, Ole Lerberg; Nielsen, Karin Michaelsen; Jensen, Svend Borup.
I: Molecules, Bind 25, Nr. 18, 4329, 09.2020.Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Attempts to Target Staphylococcus aureus Induced Osteomyelitis Bone Lesions in a Juvenile Pig Model by Using Radiotracers
AU - Afzelius, Pia
AU - Alstrup, Aage Kristian Olsen
AU - Nielsen, Ole Lerberg
AU - Nielsen, Karin Michaelsen
AU - Jensen, Svend Borup
PY - 2020/9
Y1 - 2020/9
N2 - Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.
AB - Background [18F]FDG Positron Emission Tomography cannot differentiate between sterile inflammation and infection. Therefore, we, aimed to develop more specific radiotracers fitted for differentiation between sterile and septic infection to improve the diagnostic accuracy. Consequently, the clinicians can refine the treatment of, for example, prosthesis-related infection. METHODS: We examined different target points; Staphylococcus aureus biofilm (68Ga-labeled DOTA-K-A9 and DOTA-GSGK-A11), bone remodeling ([18F]NaF), bacterial cell membranes ([68Ga]Ga-Ubiquicidin), and leukocyte trafficking ([68Ga]Ga-DOTA-Siglec-9). We compared them to the well-known glucose metabolism marker [18F]FDG, in a well-established juvenile S. aureus induced osteomyelitis (OM) pig model. RESULTS: [18F]FDG accumulated in the OM lesions seven days after bacterial inoculation, but disappointingly we were not able to identify any tracer accumulation in OM with any of the supposedly more specific tracers. CONCLUSION: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.
KW - animal experimentation
KW - osteomyelitis
KW - PET/CT
KW - pigs
KW - [18F]FDG
KW - [18F]NaF
KW - [68Ga]Ga-DOTA-GSGK-A11 [68Ga]Ga-DOTA-Siglec-9
KW - [68Ga]Ga-DOTA-K-A9
KW - [68Ga]Ga-ubiquicidin
UR - http://www.scopus.com/inward/record.url?scp=85091540099&partnerID=8YFLogxK
U2 - 10.3390/molecules25184329
DO - 10.3390/molecules25184329
M3 - Journal article
C2 - 32967275
AN - SCOPUS:85091540099
VL - 25
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 18
M1 - 4329
ER -