Astrocytes in Paper Chips and Their Interaction with Hybrid Vesicles

Cathrine Abild Meyer, Paula De Dios Andres, Edit Brodszkij, Isabella N. Westensee, Joseph Lyons, Sandra H. Vaz*, Brigitte Städler*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

71 Downloads (Pure)

Abstract

The role of astrocytes in brain function has received increased attention lately due to their critical role in brain development and function under physiological and pathophysiological conditions. However, the biological evaluation of soft material nanoparticles in astrocytes remains unexplored. Here, the interaction of crosslinked hybrid vesicles (HVs) and either C8-D1A astrocytes or primary astrocytes cultured in polystyrene tissue culture or floatable paper-based chips is investigated. The amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) (P1) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipids are used for the assembly of HVs with crosslinked membranes. The assemblies show no short-term toxicity towards the C8-D1A astrocytes and the primary astrocytes, and both cell types internalize the HVs when cultured in 2D cell culture. Further, it is demonstrated that both the C8-D1A astrocytes and the primary astrocytes could mature in paper-based chips with preserved calcium signaling and glial fibrillary acidic protein expression. Last, it is confirmed that both types of astrocytes could internalize the HVs when cultured in paper-based chips. These findings lay out a fundamental understanding of the interaction between soft material nanoparticles and astrocytes, even when primary astrocytes are cultured in paper-based chips offering a 3D environment.

OriginalsprogEngelsk
Artikelnummer2200209
TidsskriftAdvanced Biology
Vol/bind7
Nummer1
ISSN2701-0198
DOI
StatusUdgivet - jan. 2023

Fingeraftryk

Dyk ned i forskningsemnerne om 'Astrocytes in Paper Chips and Their Interaction with Hybrid Vesicles'. Sammen danner de et unikt fingeraftryk.

Citationsformater