Assessing the Effect of Magnetite Nanoflowers on Platelets in a Multiscale Approach in the Context of Thromboembolic Diseases

TY - JOUR

T1 - Assessing the Effect of Magnetite Nanoflowers on Platelets in a Multiscale Approach in the Context of Thromboembolic Diseases

AU - Colombo, Monika

AU - Meng, Yingchao

AU - Poirier-Quinot, Marie

AU - Rollet, Anne Laure

AU - Geeverding, Audrey

AU - Delcea, Mihaela

AU - deMello, Andrew J.

AU - Abou-Hassan, Ali

PY - 2024/8

Y1 - 2024/8

N2 - Iron oxide magnetic nanoparticles have emerged as promising theranostic agents for the treatment of cardiovascular diseases. Using a multiscale approach, we investigated the interaction of 26.5 nm diameter multicore magnetite nanoflowers, recognized for being excellent nanoheaters and contrast agents in nanomedicine, with human blood platelets. Using flow cytometry, we determined safe concentrations of magnetite nanoflowers. Data indicated that platelets exhibit reversible activation at high magnetite nanoflower concentrations. Magnetic resonance imaging revealed significant reductions in T1 and T2 relaxation times when platelets were combined with magnetite nanoflowers at high concentrations suggesting nanoparticle-platelet interaction. At the nanoscale, transmission and scanning electron microscopy confirmed morphological changes in platelets when exposed to a high concentration of nanoparticles and their partial internalization in the platelets. Overall, the findings support the theranostic potential of magnetite nanoflowers in whole blood for thromboembolic disease management, with the potential for future investigations over longer exposure.

AB - Iron oxide magnetic nanoparticles have emerged as promising theranostic agents for the treatment of cardiovascular diseases. Using a multiscale approach, we investigated the interaction of 26.5 nm diameter multicore magnetite nanoflowers, recognized for being excellent nanoheaters and contrast agents in nanomedicine, with human blood platelets. Using flow cytometry, we determined safe concentrations of magnetite nanoflowers. Data indicated that platelets exhibit reversible activation at high magnetite nanoflower concentrations. Magnetic resonance imaging revealed significant reductions in T1 and T2 relaxation times when platelets were combined with magnetite nanoflowers at high concentrations suggesting nanoparticle-platelet interaction. At the nanoscale, transmission and scanning electron microscopy confirmed morphological changes in platelets when exposed to a high concentration of nanoparticles and their partial internalization in the platelets. Overall, the findings support the theranostic potential of magnetite nanoflowers in whole blood for thromboembolic disease management, with the potential for future investigations over longer exposure.

KW - blood

KW - magnetite nanoflowers

KW - nanobiointeractions

KW - platelet activation

KW - vascular diseases

UR - https://www.scopus.com/pages/publications/85202704962

U2 - 10.1021/acsanm.4c02715

DO - 10.1021/acsanm.4c02715

M3 - Journal article

AN - SCOPUS:85202704962

SN - 2574-0970

VL - 7

SP - 20085

EP - 20093

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

IS - 17

ER -