Assessing metabolic rates in zebrafish using a 3D-printed intermittent-flow respirometer and swim tunnel system

Rasmus Hejlesen; Freja Burkarl Scheffler; Clara Garfiel Byrge; Kasper Kjær-Sørensen; Claus Oxvig; Angela Fago; Hans Malte

doi:10.1242/bio.060375

Assessing metabolic rates in zebrafish using a 3D-printed intermittent-flow respirometer and swim tunnel system

Rasmus Hejlesen, Freja Burkarl Scheffler, Clara Garfiel Byrge, Kasper Kjær-Sørensen, Claus Oxvig, Angela Fago^*, Hans Malte^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Zebrafish have become a widely used vertebrate model in physiology
and reliable measures of their metabolic rate are needed. We have
developed a 3D-printed respirometer and swim tunnel system and
used it for obtaining accurate measurement of standard metabolic
rate (SMR) and maximal, aerobic metabolic rate (MMR) in zebrafish
under rest and maximal exercise, respectively. We compared a slow
(stepwise) protocol to a fast (continuous) protocol for determining
MMR. The fast protocol yielded slightly (but not significantly) higher
oxygen consumption rates than the slow protocol and the data, in
contrast to the slow protocol, followed a normal distribution. These
findings point to the fast protocol as a fast and reliable method for
obtaining accurate values of MMR in zebrafish. We make the 3D
drawings for printing the system available to researchers, to help
streamline the field of metabolic research in zebrafish and other
smaller fish species.

Originalsprog	Engelsk
Artikelnummer	bio060375
Tidsskrift	Biology Open
Vol/bind	13
Nummer	6
Antal sider	6
DOI	https://doi.org/10.1242/bio.060375
Status	Udgivet - jun. 2024

Emneord

Aerobic scope
Fish
Maximal metabolic rate
Respirometer
Standard metabolic rate
respirometry

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10.1242/bio.060375Licens: CC BY

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Link to publication in Scopus

Citationsformater

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abstract = "Zebrafish have become a widely used vertebrate model in physiologyand reliable measures of their metabolic rate are needed. We havedeveloped a 3D-printed respirometer and swim tunnel system andused it for obtaining accurate measurement of standard metabolicrate (SMR) and maximal, aerobic metabolic rate (MMR) in zebrafishunder rest and maximal exercise, respectively. We compared a slow(stepwise) protocol to a fast (continuous) protocol for determiningMMR. The fast protocol yielded slightly (but not significantly) higheroxygen consumption rates than the slow protocol and the data, incontrast to the slow protocol, followed a normal distribution. Thesefindings point to the fast protocol as a fast and reliable method forobtaining accurate values of MMR in zebrafish. We make the 3Ddrawings for printing the system available to researchers, to helpstreamline the field of metabolic research in zebrafish and othersmaller fish species.",

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year = "2024",

month = jun,

doi = "10.1242/bio.060375",

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journal = "Biology Open",

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Assessing metabolic rates in zebrafish using a 3D-printed intermittent-flow respirometer and swim tunnel system. / Hejlesen, Rasmus; Scheffler, Freja Burkarl; Byrge, Clara Garfiel et al.
I: Biology Open, Bind 13, Nr. 6, bio060375, 06.2024.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

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AU - Oxvig, Claus

AU - Fago, Angela

AU - Malte, Hans

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