Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species

Morgane Touzot; Adeline Dumet; Jean Secondi; Thierry Lengagne; Hélène Henri; Emmanuel Desouhant; Claude Duchamp; Nathalie Mondy

doi:10.1016/j.cbpa.2023.111386

Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species

Morgane Touzot^*, Adeline Dumet, Jean Secondi, Thierry Lengagne, Hélène Henri, Emmanuel Desouhant, Claude Duchamp, Nathalie Mondy

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

2 Citationer (Scopus)

Abstract

The worldwide expansion of artificial light at night (ALAN) is acknowledged as a threat to biodiversity through alterations of the natural photoperiod triggering the disruption of physiological functions. In vertebrates, melatonin production during the dark phase can be decreased or suppressed by nocturnal light as shown in many taxa. But the effect of ALAN at low intensity mimicking light pollution in peri-urban area has never been investigated in amphibians. We filled this gap by studying the impact of low ALAN levels on the expression of genes related to melatonin synthesis and signaling in two anurans (agile frog, Rana dalmatina, and common toad, Bufo bufo). Circadian expression of genes encoding enzymes catalyzing melatonin synthesis (aralkylamine N-acetyltransferase, AANAT and acetylserotonin O-methyltransferase, ASMT) or melatonin receptors (Mel1a, Mel1b and Mel1c) was investigated using RT-qPCR after 23 days of nocturnal exposure to control (< 0.01 lx) or low ALAN (3 lx). We showed that the relative abundance of most transcripts was low in late afternoon and early evening (06 pm and 08 pm) and increased throughout the night in R. dalmatina. However, a clear and ample nocturnal pattern of target gene expression was not detected in control tadpoles of both species. Surprisingly, a low ALAN level had little influence on the relative expression of most melatonin-related genes. Only Mel1c expression in R. dalmatina and Mel1b expression in B. bufo were affected by ALAN. This target gene approach provides experimental evidence that melatonin signaling pathway was slightly affected by low ALAN level in anuran tadpoles.

Originalsprog	Engelsk
Artikelnummer	111386
Tidsskrift	Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Vol/bind	280
Antal sider	10
ISSN	1095-6433
DOI	https://doi.org/10.1016/j.cbpa.2023.111386
Status	Udgivet - jun. 2023

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10.1016/j.cbpa.2023.111386

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Touzot, M., Dumet, A., Secondi, J., Lengagne, T., Henri, H., Desouhant, E., Duchamp, C., & Mondy, N. (2023). Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species. Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, 280, Artikel 111386. https://doi.org/10.1016/j.cbpa.2023.111386

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title = "Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species",

abstract = "The worldwide expansion of artificial light at night (ALAN) is acknowledged as a threat to biodiversity through alterations of the natural photoperiod triggering the disruption of physiological functions. In vertebrates, melatonin production during the dark phase can be decreased or suppressed by nocturnal light as shown in many taxa. But the effect of ALAN at low intensity mimicking light pollution in peri-urban area has never been investigated in amphibians. We filled this gap by studying the impact of low ALAN levels on the expression of genes related to melatonin synthesis and signaling in two anurans (agile frog, Rana dalmatina, and common toad, Bufo bufo). Circadian expression of genes encoding enzymes catalyzing melatonin synthesis (aralkylamine N-acetyltransferase, AANAT and acetylserotonin O-methyltransferase, ASMT) or melatonin receptors (Mel1a, Mel1b and Mel1c) was investigated using RT-qPCR after 23 days of nocturnal exposure to control (< 0.01 lx) or low ALAN (3 lx). We showed that the relative abundance of most transcripts was low in late afternoon and early evening (06 pm and 08 pm) and increased throughout the night in R. dalmatina. However, a clear and ample nocturnal pattern of target gene expression was not detected in control tadpoles of both species. Surprisingly, a low ALAN level had little influence on the relative expression of most melatonin-related genes. Only Mel1c expression in R. dalmatina and Mel1b expression in B. bufo were affected by ALAN. This target gene approach provides experimental evidence that melatonin signaling pathway was slightly affected by low ALAN level in anuran tadpoles.",

keywords = "Amphibian, Anthropogenic light pollution, Gene expression, Melatonin receptors, Night-time rhythm",

author = "Morgane Touzot and Adeline Dumet and Jean Secondi and Thierry Lengagne and H{\'e}l{\`e}ne Henri and Emmanuel Desouhant and Claude Duchamp and Nathalie Mondy",

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year = "2023",

month = jun,

doi = "10.1016/j.cbpa.2023.111386",

language = "English",

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Touzot, M, Dumet, A, Secondi, J, Lengagne, T, Henri, H, Desouhant, E, Duchamp, C & Mondy, N 2023, 'Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species', Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, bind 280, 111386. https://doi.org/10.1016/j.cbpa.2023.111386

Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species. / Touzot, Morgane; Dumet, Adeline; Secondi, Jean et al.
I: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Bind 280, 111386, 06.2023.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species

AU - Touzot, Morgane

AU - Dumet, Adeline

AU - Secondi, Jean

AU - Lengagne, Thierry

AU - Henri, Hélène

AU - Desouhant, Emmanuel

AU - Duchamp, Claude

AU - Mondy, Nathalie

PY - 2023/6

Y1 - 2023/6

N2 - The worldwide expansion of artificial light at night (ALAN) is acknowledged as a threat to biodiversity through alterations of the natural photoperiod triggering the disruption of physiological functions. In vertebrates, melatonin production during the dark phase can be decreased or suppressed by nocturnal light as shown in many taxa. But the effect of ALAN at low intensity mimicking light pollution in peri-urban area has never been investigated in amphibians. We filled this gap by studying the impact of low ALAN levels on the expression of genes related to melatonin synthesis and signaling in two anurans (agile frog, Rana dalmatina, and common toad, Bufo bufo). Circadian expression of genes encoding enzymes catalyzing melatonin synthesis (aralkylamine N-acetyltransferase, AANAT and acetylserotonin O-methyltransferase, ASMT) or melatonin receptors (Mel1a, Mel1b and Mel1c) was investigated using RT-qPCR after 23 days of nocturnal exposure to control (< 0.01 lx) or low ALAN (3 lx). We showed that the relative abundance of most transcripts was low in late afternoon and early evening (06 pm and 08 pm) and increased throughout the night in R. dalmatina. However, a clear and ample nocturnal pattern of target gene expression was not detected in control tadpoles of both species. Surprisingly, a low ALAN level had little influence on the relative expression of most melatonin-related genes. Only Mel1c expression in R. dalmatina and Mel1b expression in B. bufo were affected by ALAN. This target gene approach provides experimental evidence that melatonin signaling pathway was slightly affected by low ALAN level in anuran tadpoles.

AB - The worldwide expansion of artificial light at night (ALAN) is acknowledged as a threat to biodiversity through alterations of the natural photoperiod triggering the disruption of physiological functions. In vertebrates, melatonin production during the dark phase can be decreased or suppressed by nocturnal light as shown in many taxa. But the effect of ALAN at low intensity mimicking light pollution in peri-urban area has never been investigated in amphibians. We filled this gap by studying the impact of low ALAN levels on the expression of genes related to melatonin synthesis and signaling in two anurans (agile frog, Rana dalmatina, and common toad, Bufo bufo). Circadian expression of genes encoding enzymes catalyzing melatonin synthesis (aralkylamine N-acetyltransferase, AANAT and acetylserotonin O-methyltransferase, ASMT) or melatonin receptors (Mel1a, Mel1b and Mel1c) was investigated using RT-qPCR after 23 days of nocturnal exposure to control (< 0.01 lx) or low ALAN (3 lx). We showed that the relative abundance of most transcripts was low in late afternoon and early evening (06 pm and 08 pm) and increased throughout the night in R. dalmatina. However, a clear and ample nocturnal pattern of target gene expression was not detected in control tadpoles of both species. Surprisingly, a low ALAN level had little influence on the relative expression of most melatonin-related genes. Only Mel1c expression in R. dalmatina and Mel1b expression in B. bufo were affected by ALAN. This target gene approach provides experimental evidence that melatonin signaling pathway was slightly affected by low ALAN level in anuran tadpoles.

KW - Amphibian

KW - Anthropogenic light pollution

KW - Gene expression

KW - Melatonin receptors

KW - Night-time rhythm

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U2 - 10.1016/j.cbpa.2023.111386

DO - 10.1016/j.cbpa.2023.111386

M3 - Journal article

C2 - 36740169

AN - SCOPUS:85148375090

SN - 1095-6433

VL - 280

JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

M1 - 111386

ER -

Artificial light at night triggers slight transcriptomic effects on melatonin signaling but not synthesis in tadpoles of two anuran species

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