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Analysis of barley mutants ert-c.1 and ert-d.7 reveals two loci with additive effect on plant architecture

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  • Qiongxian Lu, Carlsberg Research Center
  • ,
  • Christoph Dockter, Carlsberg Research Center
  • ,
  • Nick Sirijovski, Lund University
  • ,
  • Shakhira Zakhrabekova, Lund University
  • ,
  • Udda Lundqvist, Nordic Genetic Resource Centre (NordGen)
  • ,
  • Per L. Gregersen
  • Mats Hansson, Lund University

Main conclusion: Both mutant ert-c.1 and ert-d.7 carry T2-T3 translocations in the Ert-c gene. Principal coordinate analyses revealed the translocation types and translocation breakpoints. Mutant ert-d.7 is an Ert-cErt-d double mutant. Abstract: Mutations in the Ert-c and Ert-d loci are among the most common barley mutations affecting plant architecture. The mutants have various degrees of erect and compact spikes, often accompanied with short and stiff culms. In the current study, complementation tests, linkage mapping, principal coordinate analyses and fine mapping were conducted. We conclude that the original ert-d.7 mutant does not only carry an ert-d mutation but also an ert-c mutation. Combined, mutations in Ert-c and Ert-d cause a pyramid-dense spike phenotype, whereas mutations in only Ert-c or Ert-d give a pyramid and dense phenotype, respectively. Associations between the Ert-c gene and T2-T3 translocations were detected in both mutant ert-c.1 and ert-d.7. Different genetic association patterns indicate different translocation breakpoints in these two mutants. Principal coordinate analysis based on genetic distance and screening of recombinants from all four ends of polymorphic regions was an efficient way to narrow down the region of interest in translocation-involved populations. The Ert-c gene was mapped to the marker interval of 2_0801to1_0224 on 3HL near the centromere. The results illuminate a complex connection between two single genes having additive effects on barley spike architecture and will facilitate the identification of the Ert-c and Ert-d genes.

OriginalsprogEngelsk
Artikelnummer9
TidsskriftPlanta
Vol/bind254
Nummer1
Antal sider15
ISSN0032-0935
DOI
StatusUdgivet - 20 jun. 2021

Bibliografisk note

Funding Information:
This work was financially supported by the Green Development and Demonstration Programme (GUDP) at the Ministry of Environment and Food of Denmark, project no. 34009-12-0522, the Carlsberg Foundation, The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS 2018-01026), The Swedish Research Council (VR 2018-05117), The Erik Philip-Sörensen's Foundation and The Nilsson-Ehle Foundation.

Publisher Copyright:
© 2021, The Author(s).

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