An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Hans Christian Høiberg; Steffen Møller Sparvath; Veronica Liv Andersen; Jørgen Kjems; Ebbe Sloth Andersen

doi:10.1002/biot.201700634

An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Hans Christian Høiberg, Steffen Møller Sparvath, Veronica Liv Andersen, Jørgen Kjems, Ebbe Sloth Andersen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

Originalsprog	Engelsk
Artikelnummer	1700634
Tidsskrift	Biotechnology Journal
Vol/bind	14
Nummer	1
ISSN	1860-6768
DOI	https://doi.org/10.1002/biot.201700634
Status	Udgivet - jan. 2019

Adgang til dokumentet

10.1002/biot.201700634

Andre filer og links

Link to publication in Scopus

Citationsformater

@article{c89ca5d69d104c3588519f585c0045c5,

title = "An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown",

abstract = "The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.",

keywords = "RNA design, RNA interference, RNA nanotechnology, RNA origami, self-assembly",

author = "H{\o}iberg, {Hans Christian} and Sparvath, {Steffen M{\o}ller} and Andersen, {Veronica Liv} and J{\o}rgen Kjems and Andersen, {Ebbe Sloth}",

year = "2019",

month = jan,

doi = "10.1002/biot.201700634",

language = "English",

volume = "14",

journal = "Biotechnology Journal",

issn = "1860-6768",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

number = "1",

}

TY - JOUR

T1 - An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

AU - Høiberg, Hans Christian

AU - Sparvath, Steffen Møller

AU - Andersen, Veronica Liv

AU - Kjems, Jørgen

AU - Andersen, Ebbe Sloth

PY - 2019/1

Y1 - 2019/1

N2 - The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

AB - The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.

KW - RNA design

KW - RNA interference

KW - RNA nanotechnology

KW - RNA origami

KW - self-assembly

UR - http://www.scopus.com/inward/record.url?scp=85057280978&partnerID=8YFLogxK

U2 - 10.1002/biot.201700634

DO - 10.1002/biot.201700634

M3 - Journal article

C2 - 29802763

SN - 1860-6768

VL - 14

JO - Biotechnology Journal

JF - Biotechnology Journal

IS - 1

M1 - 1700634

ER -

An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater