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An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum

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  • Interdisciplinary Nanoscience Center
  • Institut for Kemi
An electrochemical RNA aptamer-based biosensor for rapid and label-free detection of the bronchodilator theophylline was developed. The 5'-disulfide-functionalized end of the RNA aptamer sequence was immobilized on a gold electrode, and the 3'-amino-functionalized end was conjugated with a ferrocene (Fc) redox probe. Upon binding of theophylline the aptamer switches conformation from an open unfolded state to a closed hairpin-type conformation, resulting in the increased electron-transfer efficiency between Fc and the electrode. The electrochemical response, which was measured by differential pulse voltammetry, reaches saturation within a few minutes after addition of theophylline, and the dynamic range for detecting theophylline is 0.2-10 muM. The electrode displays an inhibited response when applied directly in serum samples treated with RNase inhibitors; however a full response to the theophylline serum concentration was obtained by transferring the electrode to blank serum-free buffer solutions. It was demonstrated that theophylline is detected with high selectivity in the presence of caffeine and theobromine.
OriginalsprogEngelsk
TidsskriftJournal of the American Chemical Society
Vol/bind130
Nummer13
Sider (fra-til)4256-4258
Antal sider2
ISSN0002-7863
DOI
StatusUdgivet - 2 apr. 2008

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