Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases. / Matamoros, Manuel A; Cutrona, Maria C; Wienkoop, Stefanie; Begara-Morales, Juan C; Sandal, Niels; Orera, Irene; Barroso, Juan B; Stougaard, Jens; Becana, Manuel.

I: Plant and Cell Physiology, Bind 61, Nr. 1, 01.01.2020, s. 105-117.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Matamoros, MA, Cutrona, MC, Wienkoop, S, Begara-Morales, JC, Sandal, N, Orera, I, Barroso, JB, Stougaard, J & Becana, M 2020, 'Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases', Plant and Cell Physiology, bind 61, nr. 1, s. 105-117. https://doi.org/10.1093/pcp/pcz182

APA

Matamoros, M. A., Cutrona, M. C., Wienkoop, S., Begara-Morales, J. C., Sandal, N., Orera, I., Barroso, J. B., Stougaard, J., & Becana, M. (2020). Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases. Plant and Cell Physiology, 61(1), 105-117. https://doi.org/10.1093/pcp/pcz182

CBE

Matamoros MA, Cutrona MC, Wienkoop S, Begara-Morales JC, Sandal N, Orera I, Barroso JB, Stougaard J, Becana M. 2020. Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases. Plant and Cell Physiology. 61(1):105-117. https://doi.org/10.1093/pcp/pcz182

MLA

Vancouver

Matamoros MA, Cutrona MC, Wienkoop S, Begara-Morales JC, Sandal N, Orera I o.a. Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases. Plant and Cell Physiology. 2020 jan 1;61(1):105-117. https://doi.org/10.1093/pcp/pcz182

Author

Matamoros, Manuel A ; Cutrona, Maria C ; Wienkoop, Stefanie ; Begara-Morales, Juan C ; Sandal, Niels ; Orera, Irene ; Barroso, Juan B ; Stougaard, Jens ; Becana, Manuel. / Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases. I: Plant and Cell Physiology. 2020 ; Bind 61, Nr. 1. s. 105-117.

Bibtex

@article{48ebfa769608417083cf59e96c726aed,
title = "Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases",
abstract = "Nitric oxide (NO) is a crucial signaling molecule that conveys its bioactivity mainly through protein S-nitrosylation. This is a reversible post-translational modification (PTM) that may affect protein function. S-nitrosoglutathione (GSNO) is a cellular NO reservoir and NO donor in protein S-nitrosylation. The enzyme S-nitrosoglutathione reductase (GSNOR) degrades GSNO, thereby regulating indirectly signaling cascades associated to this PTM. Here the two GSNORs of the legume Lotus japonicus, LjGSNOR1 and LjGSNOR2, have been functionally characterized. The LjGSNOR1 gene is very active in leaves and roots, whereas LjGSNOR2 is highly expressed in nodules. The enzyme activities are regulated in vitro by redox-based PTMs. Reducing conditions and hydrogen sulfide-mediated cysteine persulfidation induced both activities, whereas cysteine oxidation or glutathionylation inhibited them. Ljgsnor1 knockout mutants contained higher levels of S-nitrosothiols. Affinity chromatography and subsequent shotgun proteomics allowed us to identify 19 proteins that are differentially S-nitrosylated in the mutant and the wild-type. These include proteins involved in biotic stress, protein degradation, antioxidant protection and photosynthesis. We propose that, in the mutant plants, deregulated protein S-nitrosylation contributes to developmental alterations, such as growth inhibition, impaired nodulation and delayed flowering and fruiting. Our results highlight the importance of GSNOR function in legume biology.",
author = "Matamoros, {Manuel A} and Cutrona, {Maria C} and Stefanie Wienkoop and Begara-Morales, {Juan C} and Niels Sandal and Irene Orera and Barroso, {Juan B} and Jens Stougaard and Manuel Becana",
note = "{\textcopyright} The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.",
year = "2020",
month = jan,
day = "1",
doi = "10.1093/pcp/pcz182",
language = "English",
volume = "61",
pages = "105--117",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Altered plant and nodule development and protein S-nitrosylation in Lotus japonicus mutants deficient in S-nitrosoglutathione reductases

AU - Matamoros, Manuel A

AU - Cutrona, Maria C

AU - Wienkoop, Stefanie

AU - Begara-Morales, Juan C

AU - Sandal, Niels

AU - Orera, Irene

AU - Barroso, Juan B

AU - Stougaard, Jens

AU - Becana, Manuel

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Nitric oxide (NO) is a crucial signaling molecule that conveys its bioactivity mainly through protein S-nitrosylation. This is a reversible post-translational modification (PTM) that may affect protein function. S-nitrosoglutathione (GSNO) is a cellular NO reservoir and NO donor in protein S-nitrosylation. The enzyme S-nitrosoglutathione reductase (GSNOR) degrades GSNO, thereby regulating indirectly signaling cascades associated to this PTM. Here the two GSNORs of the legume Lotus japonicus, LjGSNOR1 and LjGSNOR2, have been functionally characterized. The LjGSNOR1 gene is very active in leaves and roots, whereas LjGSNOR2 is highly expressed in nodules. The enzyme activities are regulated in vitro by redox-based PTMs. Reducing conditions and hydrogen sulfide-mediated cysteine persulfidation induced both activities, whereas cysteine oxidation or glutathionylation inhibited them. Ljgsnor1 knockout mutants contained higher levels of S-nitrosothiols. Affinity chromatography and subsequent shotgun proteomics allowed us to identify 19 proteins that are differentially S-nitrosylated in the mutant and the wild-type. These include proteins involved in biotic stress, protein degradation, antioxidant protection and photosynthesis. We propose that, in the mutant plants, deregulated protein S-nitrosylation contributes to developmental alterations, such as growth inhibition, impaired nodulation and delayed flowering and fruiting. Our results highlight the importance of GSNOR function in legume biology.

AB - Nitric oxide (NO) is a crucial signaling molecule that conveys its bioactivity mainly through protein S-nitrosylation. This is a reversible post-translational modification (PTM) that may affect protein function. S-nitrosoglutathione (GSNO) is a cellular NO reservoir and NO donor in protein S-nitrosylation. The enzyme S-nitrosoglutathione reductase (GSNOR) degrades GSNO, thereby regulating indirectly signaling cascades associated to this PTM. Here the two GSNORs of the legume Lotus japonicus, LjGSNOR1 and LjGSNOR2, have been functionally characterized. The LjGSNOR1 gene is very active in leaves and roots, whereas LjGSNOR2 is highly expressed in nodules. The enzyme activities are regulated in vitro by redox-based PTMs. Reducing conditions and hydrogen sulfide-mediated cysteine persulfidation induced both activities, whereas cysteine oxidation or glutathionylation inhibited them. Ljgsnor1 knockout mutants contained higher levels of S-nitrosothiols. Affinity chromatography and subsequent shotgun proteomics allowed us to identify 19 proteins that are differentially S-nitrosylated in the mutant and the wild-type. These include proteins involved in biotic stress, protein degradation, antioxidant protection and photosynthesis. We propose that, in the mutant plants, deregulated protein S-nitrosylation contributes to developmental alterations, such as growth inhibition, impaired nodulation and delayed flowering and fruiting. Our results highlight the importance of GSNOR function in legume biology.

U2 - 10.1093/pcp/pcz182

DO - 10.1093/pcp/pcz182

M3 - Journal article

C2 - 31529085

VL - 61

SP - 105

EP - 117

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 1

ER -