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Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss

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Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss. / Clabaut, Aline; Grare, Céline; Rolland-Valognes, Gaëlle; Letarouilly, Jean Guillaume; Bourrier, Chantal; Andersen, Thomas L.; Sikjær, Tanja; Rejnmark, Lars; Ejersted, Charlotte; Pastoureau, Philippe; Hardouin, Pierre; Sabatini, Massimo; Broux, Odile.

I: PLOS ONE, Bind 16, Nr. 1 , e0245014, 01.2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Clabaut, A, Grare, C, Rolland-Valognes, G, Letarouilly, JG, Bourrier, C, Andersen, TL, Sikjær, T, Rejnmark, L, Ejersted, C, Pastoureau, P, Hardouin, P, Sabatini, M & Broux, O 2021, 'Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss', PLOS ONE, bind 16, nr. 1 , e0245014. https://doi.org/10.1371/journal.pone.0245014

APA

Clabaut, A., Grare, C., Rolland-Valognes, G., Letarouilly, J. G., Bourrier, C., Andersen, T. L., Sikjær, T., Rejnmark, L., Ejersted, C., Pastoureau, P., Hardouin, P., Sabatini, M., & Broux, O. (2021). Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss. PLOS ONE, 16(1 ), [e0245014]. https://doi.org/10.1371/journal.pone.0245014

CBE

Clabaut A, Grare C, Rolland-Valognes G, Letarouilly JG, Bourrier C, Andersen TL, Sikjær T, Rejnmark L, Ejersted C, Pastoureau P, Hardouin P, Sabatini M, Broux O. 2021. Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss. PLOS ONE. 16(1 ):Article e0245014. https://doi.org/10.1371/journal.pone.0245014

MLA

Vancouver

Clabaut A, Grare C, Rolland-Valognes G, Letarouilly JG, Bourrier C, Andersen TL o.a. Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss. PLOS ONE. 2021 jan;16(1 ). e0245014. https://doi.org/10.1371/journal.pone.0245014

Author

Clabaut, Aline ; Grare, Céline ; Rolland-Valognes, Gaëlle ; Letarouilly, Jean Guillaume ; Bourrier, Chantal ; Andersen, Thomas L. ; Sikjær, Tanja ; Rejnmark, Lars ; Ejersted, Charlotte ; Pastoureau, Philippe ; Hardouin, Pierre ; Sabatini, Massimo ; Broux, Odile. / Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss. I: PLOS ONE. 2021 ; Bind 16, Nr. 1 .

Bibtex

@article{b81127501bb54c229ca3136485745d1f,
title = "Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss",
abstract = "Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. In this study, microarray gene expression profiling showed profound transcriptomic changes in osteoblasts following coculture and confirmed the enrichment of the adipocyte gene signature. Double immunofluorescence microscopic analyses demonstrated the coexpression of adipogenic and osteoblastic specific markers in individual cells, providing evidence for a transdifferentiation event. At the molecular level, this conversion was associated with upregulated expression levels of reprogramming genes and a decrease in the DNA methylation level. In line with these in vitro results, preliminary immunohistochemical analysis of bone sections revealed adipogenic marker expression in osteoblasts from elderly subjects. Altogether, these data suggest that osteoblast transdifferentiation could contribute to decreased bone mass upon ageing.",
author = "Aline Clabaut and C{\'e}line Grare and Ga{\"e}lle Rolland-Valognes and Letarouilly, {Jean Guillaume} and Chantal Bourrier and Andersen, {Thomas L.} and Tanja Sikj{\ae}r and Lars Rejnmark and Charlotte Ejersted and Philippe Pastoureau and Pierre Hardouin and Massimo Sabatini and Odile Broux",
note = "Publisher Copyright: {\textcopyright} 2021 Clabaut et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = jan,
doi = "10.1371/journal.pone.0245014",
language = "English",
volume = "16",
journal = "P L o S One",
issn = "1932-6203",
publisher = "public library of science",
number = "1 ",

}

RIS

TY - JOUR

T1 - Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss

AU - Clabaut, Aline

AU - Grare, Céline

AU - Rolland-Valognes, Gaëlle

AU - Letarouilly, Jean Guillaume

AU - Bourrier, Chantal

AU - Andersen, Thomas L.

AU - Sikjær, Tanja

AU - Rejnmark, Lars

AU - Ejersted, Charlotte

AU - Pastoureau, Philippe

AU - Hardouin, Pierre

AU - Sabatini, Massimo

AU - Broux, Odile

N1 - Publisher Copyright: © 2021 Clabaut et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/1

Y1 - 2021/1

N2 - Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. In this study, microarray gene expression profiling showed profound transcriptomic changes in osteoblasts following coculture and confirmed the enrichment of the adipocyte gene signature. Double immunofluorescence microscopic analyses demonstrated the coexpression of adipogenic and osteoblastic specific markers in individual cells, providing evidence for a transdifferentiation event. At the molecular level, this conversion was associated with upregulated expression levels of reprogramming genes and a decrease in the DNA methylation level. In line with these in vitro results, preliminary immunohistochemical analysis of bone sections revealed adipogenic marker expression in osteoblasts from elderly subjects. Altogether, these data suggest that osteoblast transdifferentiation could contribute to decreased bone mass upon ageing.

AB - Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. In this study, microarray gene expression profiling showed profound transcriptomic changes in osteoblasts following coculture and confirmed the enrichment of the adipocyte gene signature. Double immunofluorescence microscopic analyses demonstrated the coexpression of adipogenic and osteoblastic specific markers in individual cells, providing evidence for a transdifferentiation event. At the molecular level, this conversion was associated with upregulated expression levels of reprogramming genes and a decrease in the DNA methylation level. In line with these in vitro results, preliminary immunohistochemical analysis of bone sections revealed adipogenic marker expression in osteoblasts from elderly subjects. Altogether, these data suggest that osteoblast transdifferentiation could contribute to decreased bone mass upon ageing.

UR - http://www.scopus.com/inward/record.url?scp=85100439698&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0245014

DO - 10.1371/journal.pone.0245014

M3 - Journal article

C2 - 33497412

AN - SCOPUS:85100439698

VL - 16

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 1

M1 - e0245014

ER -