Aarhus University Seal / Aarhus Universitets segl

Abortive activity of Topoisomerase I: a challenge for genome integrity?

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review

Standard

Abortive activity of Topoisomerase I : a challenge for genome integrity? / Jakobsen, Kristoffer Pors; Andersen, Anni H.; Bjergbaek, Lotte.

I: Current Genetics, Bind 65, Nr. 5, 10.2019, s. 1141-1144.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{5fb0806c88834e4ba61125e62c1f5f89,
title = "Abortive activity of Topoisomerase I: a challenge for genome integrity?",
abstract = "Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DNA topoisomerase I (Top1) a SSB is generated, which is normally transient and rapidly resealed by the enzyme. However, several situations can stabilize a Top1-generated SSB, and this poses the risk of converting the SSB into a double strand break (DSB) if encountered by the replication machinery. A DSB is a more serious treat for cells as it can fuel chromosomal rearrangements and thus jeopardize genome stability and cause cells to become cancerous. In this perspective, we discuss the cellular consequences of Top1-generated damage during DNA replication with focus on the differences between endogenous Top1-generated damage and Top1 damage generated due to the use of the drug camptothecin.",
keywords = "Topoisomerase I, Break induced replication, DNA end resection, Camptothecin, Endogenous damage, Fork reversal, DNA TOPOISOMERASES, REPLICATION FORKS, COMPLEXES, CLEAVAGE, MAINTENANCE, BUBBLE, REPAIR, STRAND, SITE, TOP1",
author = "Jakobsen, {Kristoffer Pors} and Andersen, {Anni H.} and Lotte Bjergbaek",
year = "2019",
month = oct,
doi = "10.1007/s00294-019-00984-w",
language = "English",
volume = "65",
pages = "1141--1144",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Abortive activity of Topoisomerase I

T2 - a challenge for genome integrity?

AU - Jakobsen, Kristoffer Pors

AU - Andersen, Anni H.

AU - Bjergbaek, Lotte

PY - 2019/10

Y1 - 2019/10

N2 - Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DNA topoisomerase I (Top1) a SSB is generated, which is normally transient and rapidly resealed by the enzyme. However, several situations can stabilize a Top1-generated SSB, and this poses the risk of converting the SSB into a double strand break (DSB) if encountered by the replication machinery. A DSB is a more serious treat for cells as it can fuel chromosomal rearrangements and thus jeopardize genome stability and cause cells to become cancerous. In this perspective, we discuss the cellular consequences of Top1-generated damage during DNA replication with focus on the differences between endogenous Top1-generated damage and Top1 damage generated due to the use of the drug camptothecin.

AB - Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DNA topoisomerase I (Top1) a SSB is generated, which is normally transient and rapidly resealed by the enzyme. However, several situations can stabilize a Top1-generated SSB, and this poses the risk of converting the SSB into a double strand break (DSB) if encountered by the replication machinery. A DSB is a more serious treat for cells as it can fuel chromosomal rearrangements and thus jeopardize genome stability and cause cells to become cancerous. In this perspective, we discuss the cellular consequences of Top1-generated damage during DNA replication with focus on the differences between endogenous Top1-generated damage and Top1 damage generated due to the use of the drug camptothecin.

KW - Topoisomerase I

KW - Break induced replication

KW - DNA end resection

KW - Camptothecin

KW - Endogenous damage

KW - Fork reversal

KW - DNA TOPOISOMERASES

KW - REPLICATION FORKS

KW - COMPLEXES

KW - CLEAVAGE

KW - MAINTENANCE

KW - BUBBLE

KW - REPAIR

KW - STRAND

KW - SITE

KW - TOP1

U2 - 10.1007/s00294-019-00984-w

DO - 10.1007/s00294-019-00984-w

M3 - Review

C2 - 31049660

VL - 65

SP - 1141

EP - 1144

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 5

ER -