A within-subject experimental design using an object location task in rats

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Object place recognition is a prominent method used to investigate spatial memory in rodents. This object place recognition memory forms the basis of the object location task. This paper provides an extensive protocol to guide the establishment of an object location task with the option of up to four repetitions using the same cohort of rats. Both weak and strong encoding protocols can be used to study short-and long-term spatial memories of varying strength and to enable the implementation of relevant memory-inhibiting or-enhancing manipulations. In addition, repetition of the test with the counterbalancing presented here allows the combination of results from two or more tests for within-subject comparison to reduce variability between rats. This method helps to increase statistical power and is strongly recommended, particularly when running experiments that produce high variation in individual behavior. Finally, implementation of the repeated object location task increases the efficiency of studies that involve surgical procedures by saving time and labor.

OriginalsprogEngelsk
Artikelnummere62458
TidsskriftJournal of Visualized Experiments
Vol/bind2021
Nummer171
ISSN1940-087X
DOI
StatusUdgivet - maj 2021

Bibliografisk note

Funding Information:
We would like to thank Antonios Asiminas, Dorothy Tse, Kiichi O'Hara, and David Bett for insightful comments and suggestions. This study was supported by Erasmus+ (to G.B. and L.N.); the Graduate School of Health, Aarhus University (to K.H.); Novo Nordisk Foundation Young Investigator Award 2017 (NNF17OC0026774), Lundbeckfonden (DANDRITE-R248-2016-2518) and PROMEMO - Center for Proteins in Memory, a Center of Excellence funded by the Danish National Research Foundation (DNRF133) (to T.T.).

Funding Information:
We would like to thank Antonios Asiminas, Dorothy Tse, Kiichi O'Hara, and David Bett for insightful comments and suggestions. This study was supported by Erasmus+ (to G.B. and L.N.); the Graduate School of Health, Aarhus University (to K.H.); Novo Nordisk Foundation Young Investigator Award 2017 (NNF17OC0026774), Lundbeckfonden (DANDRITE-R248-2016-2518) and PROMEMO-Center for Proteins in Memory, a Center of Excellence funded by the Danish National Research Foundation (DNRF133) (to T.T.).

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