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A whole-cell process for the production of ε-caprolactone in aqueous media

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  • Vishnu S.T.Srinivasamurthy, Universität Greifswald
  • ,
  • Dominique Böttcher, Universität Greifswald
  • ,
  • Jennifer Engel
  • ,
  • Selin Kara
  • Uwe T. Bornscheuer, Universität Greifswald

ε-Caprolactone is an industrially important intermediate produced in multi-10,000 ton scale annually with broad applications. We report on a whole-cell biocatalytic conversion of cyclohexanol to ε-caprolactone using the combination of alcohol dehydrogenase (ADH) with two stability-improved variants (QM and M15) of the Baeyer-Villiger monooxygenase CHMO with a special focus on process development at the 200 mM scale. Influence of parameters such as volumetric mass transfer co-efficient, stirrer speed and catalytic loading (amount of E. coli whole-cells expressing ADH and CHMO) on the process efficiency were studied and optimised. This resulted in over 98% conversion, a product titer of 20 g L –1 and an isolated product amount of 9.1 g (80%). This corresponds to a space-time yield of 1.1 g L –1 h −1 and a reaction yield (mole of product per mole substrate) of 0.9. Comparing the two CHMO variants a significant difference in catalytic yield (weight of product to weight of catalyst; 0.6 vs 0.3) was observed without any inherent changes in the process. Hence, the reported process can accommodate in the future improved variants of the CHMO.

TidsskriftProcess Biochemistry
Sider (fra-til)22-30
Antal sider9
StatusUdgivet - 2020

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