A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Aristotelis Azariadis, Olga Agata Andrzejczak*, Frida M. Carlsen, Ida Westberg, Henrik Brinch-Pedersen, Bent L. Pedersen, Kim Hebelstrup*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants. We use ribonucleoproteins, consisting of Cas9 and sgRNA, assembled in vitro, to target a gene belonging to the nitrate and peptide transporter family. Four different sgRNAs were designed and we observed efficiency in gene-editing in the protoplast pool between 8.5% and 12.4%. Twenty-one plants were re-generated from microcalli developed from individual protoplasts. In three of the plants we found that the target gene had been edited. Two of the edited plants had deletion mutations introduced into both alleles, whereas one only had a mutation in one of the alleles. Our work demonstrates that protocols for the transformation of Solanum tuberosum can be optimized to be applied to a wild Solanum species.
OriginalsprogEngelsk
Artikelnummer1044
TidsskriftPlants
Vol/bind13
Nummer7
Antal sider11
ISSN2223-7747
DOI
StatusUdgivet - apr. 2024

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