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A Traceless Aryl-Triazene Linker for DNA-Directed Chemistry

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  • Christian Hejesen, Danmark
  • Lars Kolster Pedersen, Vipergen, Danmark
  • Kurt Vesterager Gothelf
  • Nils Jakob Vest Hansen, Vipergen, Danmark
DNA-directed synthesis of encoded combinatorial libraries of small organic compounds most often involves transfer of organic building blocks from one DNA strand to another. This requires cleavable linkers to enable cleavage of the link to the original DNA strand from which the building block is transferred. Relatively few cleavable linkers are available for DNA-directed synthesis and most often they leave an amino group at the organic molecule. Here we have extended the application of 10 aryltriazenes as traceless linkers for DNA-directed synthesis. After reaction of one building block with a building block at another DNA strand the triazene linker is cleaved and reduced with hypophosphorous acid in high yield to leave the arylgroup with a hydrogen in place of the triazene i.e. without a functional group trace. It was also demonstrated that alternatively the triazene could be converted to an azide, which was used in a cycloaddition reaction. The linker is generally stable at pH >7 and could be stored for 15 several months in a freezer without significant degradation.
OriginalsprogEngelsk
TidsskriftOrganic & Biomolecular Chemistry
Vol/bind11
Nummer15
Sider (fra-til)2493-2497
Antal sider5
ISSN1477-0520
DOI
StatusUdgivet - 21 apr. 2013

    Forskningsområder

  • Cleavable linker, Traceless linker, DNA-directed chemsitry

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