A Systematic Review of Animal Models of Disuse-Induced Bone Loss

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A Systematic Review of Animal Models of Disuse-Induced Bone Loss. / Brent, Mikkel Bo; Brüel, Annemarie; Thomsen, Jesper Skovhus.

I: Calcified Tissue International, 2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review

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@article{88814d4028bb478a9715b3e78f537e5e,
title = "A Systematic Review of Animal Models of Disuse-Induced Bone Loss",
abstract = "Objective: Several different animal models are used to study disuse-induced bone loss. This systematic review aims to give a comprehensive overview of the animal models of disuse-induced bone loss and provide a detailed narrative synthesis of each unique animal model. Methods: PubMed and Embase were systematically searched for animal models of disuse from inception to November 30, 2019. In addition, Google Scholar and personal file archives were searched for relevant publications not indexed in PubMed or Embase. Two reviewers independently reviewed titles and abstracts for full-text inclusion. Data were extracted using a predefined extraction scheme to ensure standardization. Results: 1964 titles and abstracts were screened of which 653 full-text articles were included. The most common animal species used to model disuse were rats (59%) and mice (30%). Males (53%) where used in the majority of the studies and genetically modified animals accounted for 7%. Twelve different methods to induce disuse were identified. The most frequently used methods were hindlimb unloading (44%), neurectomy (15%), bandages and orthoses (15%), and botulinum toxin (9%). The median time of disuse was 21 days (quartiles: 14 days, 36 days) and the median number of animals per group subjected to disuse was 10 (quartiles: 7, 14). Random group allocation was reported in 43% of the studies. Fewer than 5% of the studies justified the number of animals per group by a sample size calculation to ensure adequate statistical power. Conclusion: Multiple animal models of disuse-induced bone loss exist, and several species of animals have successfully been studied. The complexity of disuse-induced bone loss warrants rigid research study designs. This systematic review emphasized the need for standardization of animal disuse research and reporting.",
keywords = "Animal models, Bone loss, Disuse, Immobilization, Mechanical unloading, SEARCH FILTER, MUSCLE ATROPHY, BOTULINUM TOXIN INJECTION, RAT MODEL, MINERAL DENSITY, INDUCED OSTEOPENIA, ISCHEMIC OSTEONECROSIS, NON-WEIGHT-BEARING, SEX-DIFFERENCES, FEMORAL-HEAD",
author = "Brent, {Mikkel Bo} and Annemarie Br{\"u}el and Thomsen, {Jesper Skovhus}",
year = "2021",
doi = "10.1007/s00223-020-00799-9",
language = "English",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York LLC",

}

RIS

TY - JOUR

T1 - A Systematic Review of Animal Models of Disuse-Induced Bone Loss

AU - Brent, Mikkel Bo

AU - Brüel, Annemarie

AU - Thomsen, Jesper Skovhus

PY - 2021

Y1 - 2021

N2 - Objective: Several different animal models are used to study disuse-induced bone loss. This systematic review aims to give a comprehensive overview of the animal models of disuse-induced bone loss and provide a detailed narrative synthesis of each unique animal model. Methods: PubMed and Embase were systematically searched for animal models of disuse from inception to November 30, 2019. In addition, Google Scholar and personal file archives were searched for relevant publications not indexed in PubMed or Embase. Two reviewers independently reviewed titles and abstracts for full-text inclusion. Data were extracted using a predefined extraction scheme to ensure standardization. Results: 1964 titles and abstracts were screened of which 653 full-text articles were included. The most common animal species used to model disuse were rats (59%) and mice (30%). Males (53%) where used in the majority of the studies and genetically modified animals accounted for 7%. Twelve different methods to induce disuse were identified. The most frequently used methods were hindlimb unloading (44%), neurectomy (15%), bandages and orthoses (15%), and botulinum toxin (9%). The median time of disuse was 21 days (quartiles: 14 days, 36 days) and the median number of animals per group subjected to disuse was 10 (quartiles: 7, 14). Random group allocation was reported in 43% of the studies. Fewer than 5% of the studies justified the number of animals per group by a sample size calculation to ensure adequate statistical power. Conclusion: Multiple animal models of disuse-induced bone loss exist, and several species of animals have successfully been studied. The complexity of disuse-induced bone loss warrants rigid research study designs. This systematic review emphasized the need for standardization of animal disuse research and reporting.

AB - Objective: Several different animal models are used to study disuse-induced bone loss. This systematic review aims to give a comprehensive overview of the animal models of disuse-induced bone loss and provide a detailed narrative synthesis of each unique animal model. Methods: PubMed and Embase were systematically searched for animal models of disuse from inception to November 30, 2019. In addition, Google Scholar and personal file archives were searched for relevant publications not indexed in PubMed or Embase. Two reviewers independently reviewed titles and abstracts for full-text inclusion. Data were extracted using a predefined extraction scheme to ensure standardization. Results: 1964 titles and abstracts were screened of which 653 full-text articles were included. The most common animal species used to model disuse were rats (59%) and mice (30%). Males (53%) where used in the majority of the studies and genetically modified animals accounted for 7%. Twelve different methods to induce disuse were identified. The most frequently used methods were hindlimb unloading (44%), neurectomy (15%), bandages and orthoses (15%), and botulinum toxin (9%). The median time of disuse was 21 days (quartiles: 14 days, 36 days) and the median number of animals per group subjected to disuse was 10 (quartiles: 7, 14). Random group allocation was reported in 43% of the studies. Fewer than 5% of the studies justified the number of animals per group by a sample size calculation to ensure adequate statistical power. Conclusion: Multiple animal models of disuse-induced bone loss exist, and several species of animals have successfully been studied. The complexity of disuse-induced bone loss warrants rigid research study designs. This systematic review emphasized the need for standardization of animal disuse research and reporting.

KW - Animal models

KW - Bone loss

KW - Disuse

KW - Immobilization

KW - Mechanical unloading

KW - SEARCH FILTER

KW - MUSCLE ATROPHY

KW - BOTULINUM TOXIN INJECTION

KW - RAT MODEL

KW - MINERAL DENSITY

KW - INDUCED OSTEOPENIA

KW - ISCHEMIC OSTEONECROSIS

KW - NON-WEIGHT-BEARING

KW - SEX-DIFFERENCES

KW - FEMORAL-HEAD

U2 - 10.1007/s00223-020-00799-9

DO - 10.1007/s00223-020-00799-9

M3 - Review

C2 - 33386477

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

ER -