A single dose of cocaine raises SV2A density in hippocampus of adolescent rats

Rachele Rossi; Simone Larsen Bærentzen; Majken Thomsen; Caroline C Real; Gregers Wegener; Rodrigo Grassi-Oliveira; Albert Gjedde; Anne Landau

doi:10.1017/neu.2023.14

A single dose of cocaine raises SV2A density in hippocampus of adolescent rats

Rachele Rossi, Simone Larsen Bærentzen, Majken Thomsen, Caroline C Real, Gregers Wegener, Rodrigo Grassi-Oliveira, Albert Gjedde, Anne Landau^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

3 Citationer (Scopus)

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Abstract

Objective Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [ ³H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [ ³H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [ ³H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [ ³H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic SV2A density after a single exposure during adolescence.

Originalsprog	Engelsk
Tidsskrift	Acta Neuropsychiatrica
Vol/bind	36
Nummer	2
Sider (fra-til)	109-117
Antal sider	9
ISSN	0924-2708
DOI	https://doi.org/10.1017/neu.2023.14
Status	Udgivet - apr. 2024

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10.1017/neu.2023.14Licens: CC BY

A single dose of cocaine raises SV2A density in hippocampus of adolescent ratsForlagets udgivne version, 962 KBLicens: CC BY

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title = "A single dose of cocaine raises SV2A density in hippocampus of adolescent rats",

abstract = "Objective Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [ 3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [ 3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [ 3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [ 3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic SV2A density after a single exposure during adolescence.",

keywords = "adolescent, autoradiography, cocaine, neuronal plasticity, rats, synaptic vesicle glycoprotein 2A (SV2A), Rats, Amygdala/drug effects, Positron-Emission Tomography, Cocaine/metabolism, Nerve Tissue Proteins/drug effects, Animals, Corpus Striatum, Hippocampus/drug effects, Membrane Glycoproteins/drug effects, Brain/metabolism",

author = "Rachele Rossi and B{\ae}rentzen, {Simone Larsen} and Majken Thomsen and Real, {Caroline C} and Gregers Wegener and Rodrigo Grassi-Oliveira and Albert Gjedde and Anne Landau",

year = "2024",

month = apr,

doi = "10.1017/neu.2023.14",

language = "English",

volume = "36",

pages = "109--117",

journal = "Acta Neuropsychiatrica",

issn = "0924-2708",

publisher = "Cambridge University Press",

number = "2",

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TY - JOUR

T1 - A single dose of cocaine raises SV2A density in hippocampus of adolescent rats

AU - Rossi, Rachele

AU - Bærentzen, Simone Larsen

AU - Thomsen, Majken

AU - Real, Caroline C

AU - Wegener, Gregers

AU - Grassi-Oliveira, Rodrigo

AU - Gjedde, Albert

AU - Landau, Anne

PY - 2024/4

Y1 - 2024/4

N2 - Objective Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [ 3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [ 3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [ 3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [ 3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic SV2A density after a single exposure during adolescence.

AB - Objective Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [ 3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [ 3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [ 3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [ 3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic SV2A density after a single exposure during adolescence.

KW - adolescent

KW - autoradiography

KW - cocaine

KW - neuronal plasticity

KW - rats

KW - synaptic vesicle glycoprotein 2A (SV2A)

KW - Rats

KW - Amygdala/drug effects

KW - Positron-Emission Tomography

KW - Cocaine/metabolism

KW - Nerve Tissue Proteins/drug effects

KW - Animals

KW - Corpus Striatum

KW - Hippocampus/drug effects

KW - Membrane Glycoproteins/drug effects

KW - Brain/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85149945229&partnerID=8YFLogxK

U2 - 10.1017/neu.2023.14

DO - 10.1017/neu.2023.14

M3 - Journal article

C2 - 36847240

SN - 0924-2708

VL - 36

SP - 109

EP - 117

JO - Acta Neuropsychiatrica

JF - Acta Neuropsychiatrica

IS - 2

ER -

A single dose of cocaine raises SV2A density in hippocampus of adolescent rats

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