A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls

Christopher J. Barnes; Maria Asplund; Maja Lisa Clausen; Linett Rasmussen; Caroline Meyer Olesen; Yasemin Topal Yüksel; Paal Skytt Andersen; Thomas Litman; Kim Holmstrøm; Lene Bay; Blaine Gabriel Fritz; Thomas Bjarnsholt; Tove Agner; Anders Johannes Hansen

doi:10.1186/s12866-023-03012-7

A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls

Christopher J. Barnes^*, Maria Asplund, Maja Lisa Clausen, Linett Rasmussen, Caroline Meyer Olesen, Yasemin Topal Yüksel, Paal Skytt Andersen, Thomas Litman, Kim Holmstrøm, Lene Bay, Blaine Gabriel Fritz, Thomas Bjarnsholt, Tove Agner, Anders Johannes Hansen

^*Corresponding author af dette arbejde

Institut for Agroøkologi - Plantepatologi og Mikrobiologi

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the ‘core microbiome’ of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.

Originalsprog	Engelsk
Artikelnummer	273
Tidsskrift	BMC Microbiology
Vol/bind	23
Nummer	1
ISSN	1471-2180
DOI	https://doi.org/10.1186/s12866-023-03012-7
Status	Udgivet - sep. 2023

Adgang til dokumentet

10.1186/s12866-023-03012-7Licens: CC BY

Andre filer og links

Link to publication in Scopus

Citationsformater

Barnes, C. J., Asplund, M., Clausen, M. L., Rasmussen, L., Olesen, C. M., Yüksel, Y. T., Andersen, P. S., Litman, T., Holmstrøm, K., Bay, L., Fritz, B. G., Bjarnsholt, T., Agner, T., & Hansen, A. J. (2023). A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls. BMC Microbiology, 23(1), Artikel 273. https://doi.org/10.1186/s12866-023-03012-7

@article{81410fb53e1e44caa599429ee06c5972,

title = "A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls",

abstract = "There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the {\textquoteleft}core microbiome{\textquoteright} of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.",

keywords = "Atopic dermatitis, Bacteria, Epidermis, Fluorescent in situ hybridisation, Skin microbiome, Staphylococcus aureus",

author = "Barnes, {Christopher J.} and Maria Asplund and Clausen, {Maja Lisa} and Linett Rasmussen and Olesen, {Caroline Meyer} and Y{\"u}ksel, {Yasemin Topal} and Andersen, {Paal Skytt} and Thomas Litman and Kim Holmstr{\o}m and Lene Bay and Fritz, {Blaine Gabriel} and Thomas Bjarnsholt and Tove Agner and Hansen, {Anders Johannes}",

note = "Publisher Copyright: {\textcopyright} 2023, BioMed Central Ltd., part of Springer Nature.",

year = "2023",

month = sep,

doi = "10.1186/s12866-023-03012-7",

language = "English",

volume = "23",

journal = "BMC Microbiology",

issn = "1471-2180",

publisher = "BioMed Central Ltd.",

number = "1",

}

Barnes, CJ, Asplund, M, Clausen, ML, Rasmussen, L, Olesen, CM, Yüksel, YT, Andersen, PS, Litman, T, Holmstrøm, K, Bay, L, Fritz, BG, Bjarnsholt, T, Agner, T & Hansen, AJ 2023, 'A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls', BMC Microbiology, bind 23, nr. 1, 273. https://doi.org/10.1186/s12866-023-03012-7

A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls. / Barnes, Christopher J.; Asplund, Maria; Clausen, Maja Lisa et al.
I: BMC Microbiology, Bind 23, Nr. 1, 273, 09.2023.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls

AU - Barnes, Christopher J.

AU - Asplund, Maria

AU - Clausen, Maja Lisa

AU - Rasmussen, Linett

AU - Olesen, Caroline Meyer

AU - Yüksel, Yasemin Topal

AU - Andersen, Paal Skytt

AU - Litman, Thomas

AU - Holmstrøm, Kim

AU - Bay, Lene

AU - Fritz, Blaine Gabriel

AU - Bjarnsholt, Thomas

AU - Agner, Tove

AU - Hansen, Anders Johannes

PY - 2023/9

Y1 - 2023/9

N2 - There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the ‘core microbiome’ of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.

AB - There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the ‘core microbiome’ of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.

KW - Atopic dermatitis

KW - Bacteria

KW - Epidermis

KW - Fluorescent in situ hybridisation

KW - Skin microbiome

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=85173566123&partnerID=8YFLogxK

U2 - 10.1186/s12866-023-03012-7

DO - 10.1186/s12866-023-03012-7

M3 - Journal article

C2 - 37773096

AN - SCOPUS:85173566123

SN - 1471-2180

VL - 23

JO - BMC Microbiology

JF - BMC Microbiology

IS - 1

M1 - 273

ER -

A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater