A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

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A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds. / Zou, Lijin; Luo, Yonglun; Chen, Muwan; Wang, Gang; Ding, Ming; Petersen, Charlotte Christie; Kang, Ran; Dagnaes-Hansen, Frederik; Zeng, Yuanlin; Lv, Nonghua; Ma, Qing; Le, Dang Quang Svend; Besenbacher, Flemming; Bolund, Lars; Jensen, Thomas G; Kjems, Jørgen; Pu, William T; Bünger, Cody.

I: Scientific Reports, Bind 3, 2243, 22.07.2013.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{535d94c38c244a97bd67d7ede5f9393b,
title = "A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds",
abstract = "We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.",
author = "Lijin Zou and Yonglun Luo and Muwan Chen and Gang Wang and Ming Ding and Petersen, {Charlotte Christie} and Ran Kang and Frederik Dagnaes-Hansen and Yuanlin Zeng and Nonghua Lv and Qing Ma and Le, {Dang Quang Svend} and Flemming Besenbacher and Lars Bolund and Jensen, {Thomas G} and J{\o}rgen Kjems and Pu, {William T} and Cody B{\"u}nger",
year = "2013",
month = "7",
day = "22",
doi = "10.1038/srep02243",
language = "English",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

AU - Zou, Lijin

AU - Luo, Yonglun

AU - Chen, Muwan

AU - Wang, Gang

AU - Ding, Ming

AU - Petersen, Charlotte Christie

AU - Kang, Ran

AU - Dagnaes-Hansen, Frederik

AU - Zeng, Yuanlin

AU - Lv, Nonghua

AU - Ma, Qing

AU - Le, Dang Quang Svend

AU - Besenbacher, Flemming

AU - Bolund, Lars

AU - Jensen, Thomas G

AU - Kjems, Jørgen

AU - Pu, William T

AU - Bünger, Cody

PY - 2013/7/22

Y1 - 2013/7/22

N2 - We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.

AB - We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.

U2 - 10.1038/srep02243

DO - 10.1038/srep02243

M3 - Journal article

C2 - 23873182

VL - 3

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 2243

ER -