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A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping

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A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. / Sandal, Niels; Jin, Haojie; Rodriguez-Navarro, Dulce Nombre; Temprano, Francisco; Cvitanich, Cristina; Brachmann, Andreas; Sato, Shusei; Kawaguchi, Masayoshi; Tabata, Satoshi; Parniske, Martin; Ruiz-Sainz, Jose E; Andersen, Stig Uggerhøj; Stougaard, Jens.

I: D N A Research, Bind 19, Nr. 4, 08.2012, s. 317-323.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Sandal, N, Jin, H, Rodriguez-Navarro, DN, Temprano, F, Cvitanich, C, Brachmann, A, Sato, S, Kawaguchi, M, Tabata, S, Parniske, M, Ruiz-Sainz, JE, Andersen, SU & Stougaard, J 2012, 'A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping', D N A Research, bind 19, nr. 4, s. 317-323. https://doi.org/10.1093/dnares/dss014

APA

Sandal, N., Jin, H., Rodriguez-Navarro, D. N., Temprano, F., Cvitanich, C., Brachmann, A., Sato, S., Kawaguchi, M., Tabata, S., Parniske, M., Ruiz-Sainz, J. E., Andersen, S. U., & Stougaard, J. (2012). A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. D N A Research, 19(4), 317-323. https://doi.org/10.1093/dnares/dss014

CBE

Sandal N, Jin H, Rodriguez-Navarro DN, Temprano F, Cvitanich C, Brachmann A, Sato S, Kawaguchi M, Tabata S, Parniske M, Ruiz-Sainz JE, Andersen SU, Stougaard J. 2012. A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. D N A Research. 19(4):317-323. https://doi.org/10.1093/dnares/dss014

MLA

Vancouver

Sandal N, Jin H, Rodriguez-Navarro DN, Temprano F, Cvitanich C, Brachmann A o.a. A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. D N A Research. 2012 aug;19(4):317-323. https://doi.org/10.1093/dnares/dss014

Author

Sandal, Niels ; Jin, Haojie ; Rodriguez-Navarro, Dulce Nombre ; Temprano, Francisco ; Cvitanich, Cristina ; Brachmann, Andreas ; Sato, Shusei ; Kawaguchi, Masayoshi ; Tabata, Satoshi ; Parniske, Martin ; Ruiz-Sainz, Jose E ; Andersen, Stig Uggerhøj ; Stougaard, Jens. / A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. I: D N A Research. 2012 ; Bind 19, Nr. 4. s. 317-323.

Bibtex

@article{2d48fdd2ac0a42cc8b9fac098f405ad5,
title = "A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping",
abstract = "Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.",
author = "Niels Sandal and Haojie Jin and Rodriguez-Navarro, {Dulce Nombre} and Francisco Temprano and Cristina Cvitanich and Andreas Brachmann and Shusei Sato and Masayoshi Kawaguchi and Satoshi Tabata and Martin Parniske and Ruiz-Sainz, {Jose E} and Andersen, {Stig Uggerh{\o}j} and Jens Stougaard",
year = "2012",
month = aug,
doi = "10.1093/dnares/dss014",
language = "English",
volume = "19",
pages = "317--323",
journal = "D N A Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping

AU - Sandal, Niels

AU - Jin, Haojie

AU - Rodriguez-Navarro, Dulce Nombre

AU - Temprano, Francisco

AU - Cvitanich, Cristina

AU - Brachmann, Andreas

AU - Sato, Shusei

AU - Kawaguchi, Masayoshi

AU - Tabata, Satoshi

AU - Parniske, Martin

AU - Ruiz-Sainz, Jose E

AU - Andersen, Stig Uggerhøj

AU - Stougaard, Jens

PY - 2012/8

Y1 - 2012/8

N2 - Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.

AB - Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.

U2 - 10.1093/dnares/dss014

DO - 10.1093/dnares/dss014

M3 - Journal article

C2 - 22619310

VL - 19

SP - 317

EP - 323

JO - D N A Research

JF - D N A Research

SN - 1340-2838

IS - 4

ER -