A robust method of determination of high concentrations of peptides and proteins

Pavel A Levashov, Duncan S Sutherland, Flemming Besenbacher, Stepan Shipovskov

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    Abstract

    In this paper, we pioneer application of a unique method of protein determination by coloring peptide bonds for analysis of a variety of biomolecules with different grades of purity (e.g., oligopeptides, membrane, and glycol proteins). We demonstrated that the calibration curve for all studied molecules is universal and linear within 0.1 to 1.2mg protein content range. The assay thus can be used to analyze peptides without preliminary dilutions and calibration in up to 1g/ml solutions of peptides, which is crucial for many biotechnological processes, such as development of coatings, scaffolds, and biocompatible materials.
    OriginalsprogEngelsk
    TidsskriftAnalytical Biochemistry
    Vol/bind395
    Nummer1
    Sider (fra-til)111-2
    Antal sider2
    ISSN0003-2697
    DOI
    StatusUdgivet - 1 dec. 2009

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