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A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
DOI
- https://doi.org/10.1038/s41598-022-07313-1
Forlagets udgivne version
- Benjamin B. Kim, Stanford University ,
- Haodi Wu, Stanford University, University of Pittsburgh ,
- Yukun A. Hao, Stanford University ,
- Michael Pan, Stanford University ,
- Mariya Chavarha, Stanford University ,
- Yufeng Zhao, Stanford University ,
- Michael Westberg
- François St-Pierre, Baylor College of Medicine ,
- Joseph C. Wu, Stanford University ,
- Michael Z. Lin, Stanford University
A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in the presence of sample motion. We performed combinatorial multi-site mutagenesis on a cyan-excitable red fluorescent protein to create the bright and monomeric mCyRFP3, which proved to be uniquely non-perturbing when fused to the GEVI ASAP3. The green/red ratio from ASAP3-mCyRFP3 (ASAP3-R3) reported voltage while correcting for motion artifacts, allowing the visualization of membrane voltage changes in contracting cardiomyocytes and throughout the cell cycle of motile cells.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 3678 |
| Tidsskrift | Scientific Reports |
| Vol/bind | 12 |
| Nummer | 1 |
| Antal sider | 11 |
| ISSN | 2045-2322 |
| DOI | |
| Status | Udgivet - 2022 |
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