Abstract
A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in the presence of sample motion. We performed combinatorial multi-site mutagenesis on a cyan-excitable red fluorescent protein to create the bright and monomeric mCyRFP3, which proved to be uniquely non-perturbing when fused to the GEVI ASAP3. The green/red ratio from ASAP3-mCyRFP3 (ASAP3-R3) reported voltage while correcting for motion artifacts, allowing the visualization of membrane voltage changes in contracting cardiomyocytes and throughout the cell cycle of motile cells.
Originalsprog | Engelsk |
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Artikelnummer | 3678 |
Tidsskrift | Scientific Reports |
Vol/bind | 12 |
Nummer | 1 |
Antal sider | 11 |
ISSN | 2045-2322 |
DOI | |
Status | Udgivet - 2022 |