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A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

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DOI

  • Jianping Zhang, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Timothy C Hewitt, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Willem H P Boshoff, Department of Plant Sciences, University of the Free State, Bloemfontein, South Africa, Sydafrika
  • Ian Dundas, School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, SA, Australia, Australien
  • Narayana Upadhyaya, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Jianbo Li, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, Australien
  • Mehran Patpour
  • Sutha Chandramohan, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Zacharias A Pretorius, Department of Plant Sciences, University of the Free State, Bloemfontein, South Africa, Sydafrika
  • Mogens Støvring Hovmøller
  • Wendelin Schnippenkoetter, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Robert F Park, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, Australien
  • Rohit Mago, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Sambasivam Periyannan, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Dhara Bhatt, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Sami Hoxha, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, Australien
  • Soma Chakraborty, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Ming Luo, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Peter Dodds, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Burkhard Steuernagel, John Innes Centre, Norwich Research Park, Norwich, UK, Storbritannien
  • Brande B H Wulff, John Innes Centre, Norwich Research Park, Norwich, UK, Storbritannien
  • Michael Ayliffe, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
  • Robert A McIntosh, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, Australien
  • Peng Zhang, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, Australien
  • Evans S Lagudah, Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia, CSIRO Agriculture & Food, Canberra, ACT, Australia, Australien
The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.
OriginalsprogEngelsk
Artikelnummer3378
TidsskriftNature Communications
Vol/bind12
ISSN2041-1723
DOI
StatusUdgivet - jun. 2021

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