A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirstine Kirkegaard; Johnny Juhl Hindkjær; Marie Louise Grøndahl; Ulrik Schiøler Kesmodel; Hans Jakob Ingerslev

doi:10.1007/s10815-012-9750-x

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirstine Kirkegaard, Johnny Juhl Hindkjær, Marie Louise Grøndahl, Ulrik Schiøler Kesmodel, Hans Jakob Ingerslev

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

125 Citationer (Scopus)

337 Downloads (Pure)

Abstract

PURPOSE: Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing time-lapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator.

METHODS: In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥ 8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer.

RESULTS: No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR(TLI/COI): 0.81 (0.65; 1.02)) or PP (RR(TLI/COI): 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR(TLI/COI): 0.96 (0.73; 1.26)); PP (RR(TLI/COI): 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR(TLI/COI): 1.09 (0.84; 1.41)); PP (RR(TLI/COI): 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate.

CONCLUSION: Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

Originalsprog	Engelsk
Tidsskrift	Journal of Assisted Reproduction and Genetics
Vol/bind	29
Nummer	6
Sider (fra-til)	565-572
Antal sider	8
ISSN	1058-0468
DOI	https://doi.org/10.1007/s10815-012-9750-x
Status	Udgivet - jun. 2012

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title = "A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator",

abstract = "PURPOSE: Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing time-lapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator.METHODS: In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥ 8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer.RESULTS: No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR(TLI/COI): 0.81 (0.65; 1.02)) or PP (RR(TLI/COI): 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR(TLI/COI): 0.96 (0.73; 1.26)); PP (RR(TLI/COI): 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR(TLI/COI): 1.09 (0.84; 1.41)); PP (RR(TLI/COI): 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate.CONCLUSION: Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.",

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author = "Kirstine Kirkegaard and Hindkj{\ae}r, \{Johnny Juhl\} and Gr{\o}ndahl, \{Marie Louise\} and Kesmodel, \{Ulrik Schi{\o}ler\} and Ingerslev, \{Hans Jakob\}",

year = "2012",

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doi = "10.1007/s10815-012-9750-x",

language = "English",

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A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator. / Kirkegaard, Kirstine; Hindkjær, Johnny Juhl; Grøndahl, Marie Louise et al.
I: Journal of Assisted Reproduction and Genetics, Bind 29, Nr. 6, 06.2012, s. 565-572.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

AU - Kirkegaard, Kirstine

AU - Hindkjær, Johnny Juhl

AU - Grøndahl, Marie Louise

AU - Kesmodel, Ulrik Schiøler

AU - Ingerslev, Hans Jakob

PY - 2012/6

Y1 - 2012/6

N2 - PURPOSE: Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing time-lapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator.METHODS: In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥ 8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer.RESULTS: No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR(TLI/COI): 0.81 (0.65; 1.02)) or PP (RR(TLI/COI): 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR(TLI/COI): 0.96 (0.73; 1.26)); PP (RR(TLI/COI): 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR(TLI/COI): 1.09 (0.84; 1.41)); PP (RR(TLI/COI): 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate.CONCLUSION: Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

AB - PURPOSE: Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing time-lapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator.METHODS: In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥ 8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer.RESULTS: No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR(TLI/COI): 0.81 (0.65; 1.02)) or PP (RR(TLI/COI): 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR(TLI/COI): 0.96 (0.73; 1.26)); PP (RR(TLI/COI): 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR(TLI/COI): 1.09 (0.84; 1.41)); PP (RR(TLI/COI): 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate.CONCLUSION: Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

KW - Adult

KW - Embryo Culture Techniques

KW - Embryo Implantation

KW - Female

KW - Fertilization in Vitro

KW - Humans

KW - Incubators

KW - Oocytes

KW - Pregnancy

KW - Pregnancy Rate

U2 - 10.1007/s10815-012-9750-x

DO - 10.1007/s10815-012-9750-x

M3 - Journal article

C2 - 22460082

SN - 1058-0468

VL - 29

SP - 565

EP - 572

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 6

ER -

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

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